Journal of Experimental Medicine, Vol 164, 882-896, Copyright © 1986 by Rockefeller University Press

ARTICLES

Ia molecule-associated selectivity in T cell recognition of a 23-amino- acid peptide of lysozyme

N Shastri, G Gammon, A Miller and EE Sercarz

Ak- or Ek-restricted T cells, generated by immunization with a 23-amino- acid peptide of hen egg-white lysozyme (amino acid 74-96), showed a strict correlation between the minimal peptide determinant recognized and the Ia molecule restricting recognition. All Ak-restricted clones obtained from six independently derived lines recognized determinants contained within peptides 74-86, while Ek-restricted clones recognized determinants within 85-96. This correlation was true whether B10.A mice (Ak, Ek) were immunized with peptide 74-96 or with each of the two smaller peptides (74-86 or 85-96). Furthermore, a T cell response could be obtained to peptide 74-86, but not to peptide 85-96 in B10.A (4R) mice, which express only the Ak molecule. Thus, an Ia molecule- associated selectivity exists in the choice of T cell determinants even within this small 23-amino-acid peptide antigen. Significant differences were noted, however, in the boundaries of the minimal peptide determinants recognized within peptide 74-96 by Ak- or Ek- restricted T cells, in comparison to those recognized by Ab-restricted T cells. These results indicate that interaction of the same peptide with distinct Ia molecules results in recognition of unique aspects of the antigenic determinants by the T cell receptor.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• McPherson, S. W., Roberts, J. P., Gregerson, D. S. (1999). Systemic Expression of Rat Soluble Retinal Antigen Induces Resistance to Experimental Autoimmune Uveoretinitis. J. Immunol. 163: 4269-4276 [Abstract] [Full Text]  
• Buus, S, Sette, A, Colon, S., Miles, C, Grey, H. (1987). The relation between major histocompatibility complex (MHC) restriction and the capacity of Ia to bind immunogenic peptides. Science 235: 1353-1358 [Abstract]  
• Guillet, J., Lai, M., Briner, T., Buus, S, Sette, A, Grey, H., Smith, J., Gefter, M. (1987). Immunological self, nonself discrimination. Science 235: 865-870 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS