Dissection of the lymphokine-activated killer phenomenon. Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis

doi:10.1084/jem.164.3.814

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Dissection of the lymphokine-activated killer phenomenon. Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis

JH Phillips and LL Lanier

In vitro culture of human peripheral blood lymphocytes in IL-2 results in the generation of cytotoxic cells that can lyse fresh and cultured solid tumor cells, as well as hematopoietic tumor cell lines, without deliberate immunization or MHC restriction. This has been referred to as the lymphokine activated killer (LAK) phenomenon. Here, we show that the majority of this activity is mediated by NK cells that express the Leu-19 (NKH-1) antigen, but do not express CD3. The precursor of this effector population also expressed the phenotype CD3-, Leu-19+. Peripheral blood CD3+ T lymphocytes contributed little to the LAK phenomenon, although low levels of non-MHC restricted cytotoxicity against hematopoietic tumor cell targets were mediated by a subset of CD3+ T lymphocytes that coexpressed the Leu-19 antigen. These studies clearly indicated that the LAK phenomenon is not mediated by a unique LAK cell, but is mediated mainly by IL-2-activated peripheral blood NK cells.
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Choi, S. S., Chhabra, V. S., Nguyen, Q. H., Ank, B. J., Stiehm, E. R., Roberts, R. L. (2004). Interleukin-15 Enhances Cytotoxicity, Receptor Expression, and Expansion of Neonatal Natural Killer Cells in Long-Term Culture. CVI 11: 879-888 [Abstract] [Full Text]
Atanackovic, D., Block, A., de Weerth, A., Faltz, C., Hossfeld, D. K., Hegewisch-Becker, S. (2004). Characterization of Effusion-Infiltrating T Cells: Benign versus Malignant Effusions. Clin. Cancer Res. 10: 2600-2608 [Abstract] [Full Text]
Piccioli, D., Sbrana, S., Melandri, E., Valiante, N. M. (2002). Contact-dependent Stimulation and Inhibition of Dendritic Cells by Natural Killer Cells. JEM 195: 335-341 [Abstract] [Full Text]
Falk, C. S., Noessner, E., Weiss, E. H., Schendel, D. J. (2002). Retaliation against Tumor Cells Showing Aberrant HLA Expression Using Lymphokine Activated Killer-derived T Cells. Cancer Res. 62: 480-487 [Abstract] [Full Text]
Fiorentini, S., Licenziati, S., Alessandri, G., Castelli, F., Caligaris, S., Bonafede, M., Grassi, M., Garrafa, E., Balsari, A., Turano, A., Caruso, A. (2001). CD11b Expression Identifies CD8+CD28+ T Lymphocytes with Phenotype and Function of Both Naive/Memory and Effector Cells. J. Immunol. 166: 900-907 [Abstract] [Full Text]
Brandau, S., Suttmann, H., Riemensberger, J., Seitzer, U., Arnold, J., Durek, C., Jocham, D., Flad, H.-D., Böhle, A. (2000). Perforin-mediated Lysis of Tumor Cells by Mycobacterium Bovis Bacillus Calmette-Guerin-activated Killer Cells. Clin. Cancer Res. 6: 3729-3738 [Abstract] [Full Text]
Yoneda, O., Imai, T., Goda, S., Inoue, H., Yamauchi, A., Okazaki, T., Imai, H., Yoshie, O., Bloom, E. T., Domae, N., Umehara, H. (2000). Fractalkine-Mediated Endothelial Cell Injury by NK Cells. J. Immunol. 164: 4055-4062 [Abstract] [Full Text]
Shimizu, J., Yamazaki, S., Sakaguchi, S. (1999). Induction of Tumor Immunity by Removing CD25+CD4+ T Cells: A Common Basis Between Tumor Immunity and Autoimmunity. J. Immunol. 163: 5211-5218 [Abstract] [Full Text]
Kozlowski, M., Schorey, J., Portis, T., Grigoriev, V., Kornbluth, J. (1999). NK Lytic-Associated Molecule: A Novel Gene Selectively Expressed in Cells with Cytolytic Function. J. Immunol. 163: 1775-1785 [Abstract] [Full Text]
Idris, A. H., Iizuka, K., Smith, H. R.C., Scalzo, A. A., Yokoyama, W. M. (1998). Genetic Control Of Natural Killing and In Vivo Tumor Elimination by the Chok Locus. JEM 188: 2243-2256 [Abstract] [Full Text]
Azzoni, L., Zatsepina, O., Abebe, B., Bennett, I. M., Kanakaraj, P., Perussia, B. (1998). Differential Transcriptional Regulation of CD161 and a Novel Gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T Cells. J. Immunol. 161: 3493-3500 [Abstract] [Full Text]
Matsumoto, G., Nghiem, M. P., Nozaki, N., Schmits, R., Penninger, J. M. (1998). Cooperation Between CD44 and LFA-1/CD11a Adhesion Receptors in Lymphokine-Activated Killer Cell Cytotoxicity. J. Immunol. 160: 5781-5789 [Abstract] [Full Text]
Tomura, M., Zhou, X.-Y., Maruo, S., Ahn, H.-J., Hamaoka, T., Okamura, H., Nakanishi, K., Tanimoto, T., Kurimoto, M., Fujiwara, H. (1998). A Critical Role for IL-18 in the Proliferation and Activation of NK1.1+CD3- Cells. J. Immunol. 160: 4738-4746 [Abstract] [Full Text]
Nguyen, Q. H., Roberts, R. L., Ank, B. J., Lin, S.-J., Lau, C. K., Stiehm, E. R. (1998). Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12. CVI 5: 98-104 [Abstract] [Full Text]
Porgador, A., Mandelboim, O., Restifo, N. P., Strominger, J. L. (1997). Natural killer cell lines kill autologous beta 2-microglobulin-deficient melanoma cells: Implications for cancer�immunotherapy. Proc. Natl. Acad. Sci. USA 94: 13140-13145 [Abstract] [Full Text]
Fujieda, S., Noda, I., Saito, H., Hoshino, T., Yagita, M. (1994). Swainsonine Augments the Cytotoxicity of Human Lymphokine-Activated Killer Cells Against Autologous Thyroid Cancer Cells. Arch Otolaryngol Head Neck Surg 120: 389-394 [Abstract]
Takahashi, H, Nakada, T, Puisieux, I (1993). Inhibition of human colon cancer growth by antibody-directed human LAK cells in SCID mice. Science 259: 1460-1463 [Abstract]
Johnkoski, J. A., Tzung, S.-P., Doerr, R. J., Cohen, S. A. (1992). Hepatic Metastasis Alters the Immune Function of Murine Liver Nonparenchymal Cells. Arch Surg 127: 1325-1329 [Abstract]
Stotter, H., Lotze, M. T. (1991). Human Lymphokine-Activated Killer Cell Activity: Role of IL-2, IL-4, and IL-7. Arch Surg 126: 1525-1530 [Abstract]
Wood, N. L., Kitces, E. N., Blaylock, W. K. (1990). Depressed Lymphokine Activated Killer Cell Activity in Mycosis Fungoides: A Possible Marker for Aggressive Disease. Arch Dermatol 126: 907-913 [Abstract]
Balch, C. M., Riley, L. B., Bae, Y. J., Salmeron, M. A., Platsoucas, C. D., von Eschenbach, A., Itoh, K. (1990). Patterns of Human Tumor-Infiltrating Lymphocytes in 120 Human Cancers. Arch Surg 125: 200-205 [Abstract]
Lindemann, R.A., Miyasaki, K.T., Wolinsky, L.E. (1988). Induction of Activated Lymphocyte Killing by Bacteria Associated with Periodontal Disease. JDR 67: 846-850 [Abstract]
Siegel, J., Sharon, M, Smith, P., Leonard, W. (1987). The IL-2 receptor beta chain (p70): role in mediating signals for LAK, NK, and proliferative activities. Science 238: 75-78 [Abstract]
FAHEY, J. L., SARNA, G., GALE, R. P., SEEGER, R. (1987). Immune Interventions in Disease. ANN INTERN MED 106: 257-274 [Abstract]