Journal of Experimental Medicine, Vol 163, 938-951, Copyright © 1986 by Rockefeller University Press

ARTICLES

Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes

JR Dasch and PP Jones

Long-term cultured bone marrow cells were characterized with respect to a number of B and pre-B cell markers. Cells expressing ThB, B-220, and IgM were found within cultures set up according to the procedure of Whitlock and Witte. This culture system was modified by placing sorted pre-B cells (ThB+, IgM-) from bone marrow in culture with previously- established bone marrow adherent layers. These cultures commenced growth without the lag associated with the Whitlock cultures. These cultured nonadherent cells show a high frequency of IgM+ cells, but do not express either IgD or Ia, and we refer to them as immature B cells. Cells with a similar phenotype (IgM+, Ia-, IgD-) are found within the spleens of young but not adult mice. The phorbol ester PMA induces expression of IgD on the cultured immature B cells, but has no effect on Ia expression. This suggests that the processing of H chain RNA transcripts may be affected by protein kinase C. These results demonstrate that the appearance of IgM, IgD, and Ia are independently controlled in long-term cultured B-lineage cells.
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This article has been cited by other articles:

• Lu, L.-S., Tung, J., Baumgarth, N., Herman, O., Gleimer, M., Herzenberg, L. A., Herzenberg, L. A. (2002). Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway. Proc. Natl. Acad. Sci. USA 99: 3007-3012 [Abstract] [Full Text]  

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