Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors

doi:10.1084/jem.163.3.632

This Article

	Full Text (PDF, 706K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Vilcek, J.
	Articles by Tsujimoto, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Vilcek, J.
	Articles by Tsujimoto, M.


Social Bookmarking

	What's this?

ARTICLES

Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors

J Vilcek, VJ Palombella, D Henriksen-DeStefano, C Swenson, R Feinman, M Hirai and M Tsujimoto

Tumor necrosis factor (TNF) is a monocyte-derived protein cytotoxic or cytostatic for some tumor cell lines. Here we show that highly purified E. coli-derived recombinant human TNF stimulated the growth of human FS- 4 diploid fibroblasts. Stimulation of cell growth was demonstrable at a TNF concentration of 10 pg/ml (3 X 10(-13) M). Maximal stimulation was attained at TNF concentrations of 10 ng/ml (3 X 10(-10) M) or higher. Growth-stimulatory activity of TNF was inhibited by an mAb neutralizing the cytotoxic activity of TNF. Growth stimulation was not inhibited by another mAb specific for TNF, lacking neutralizing activity for the cytotoxic activity of TNF. Growth stimulation by TNF was more marked and more sustained in the presence of greater than or equal to 10% FCS than in medium with less than or equal to 5% FCS. Addition of TNF to confluent FS-4 cultures also produced a marked stimulation of cell growth in the presence of fresh FCS, while a much less marked stimulation was seen in the absence of FCS. Stimulation of confluent cultures by TNF in serum-free medium was enhanced by insulin, suggesting that insulin or insulin-like growth factor(s) in the serum can act synergistically with TNF in producing growth stimulation. While the growth-stimulatory effects of TNF and insulin were synergistic, the actions of TNF and epidermal growth factor (EGF) were less than additive, suggesting that TNF and EGF may activate identical or similar pathways. We conclude that stimulation of cell growth is probably a physiological function of TNF, and that the cytotoxic and cytostatic actions of TNF may be the result of an anomalous growth signal transduction in neoplastic cells lacking the constraints of normal growth control mechanisms.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Williams, T J, Wilson, J W (2008). Challenges in pulmonary fibrosis: 7 {middle dot} Novel therapies and lung transplantation. Thorax 63: 277-284 [Abstract] [Full Text]
Chang, S.-J., Tsai, P.-C., Chen, C.-J., Lai, H.-M., Ko, Y.-C. (2007). The polymorphism -863C/A in tumour necrosis factor-{alpha} gene contributes an independent association to gout. Rheumatology (Oxford) 46: 1662-1666 [Abstract] [Full Text]
Saito, K., Hirokawa, M., Inaba, K., Fukaya, H., Kawabata, Y., Komatsuda, A., Yamashita, J., Sawada, K. (2006). Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in culture with thrombopoietin and tumor necrosis factor-{alpha} and its possible role in hemophagocytic syndrome. Blood 107: 1366-1374 [Abstract] [Full Text]
Lopez, C. A., Kimchi, E. T., Mauceri, H. J., Park, J. O., Mehta, N., Murphy, K. T., Beckett, M. A., Hellman, S., Posner, M. C., Kufe, D. W., Weichselbaum, R. R. (2004). Chemoinducible gene therapy: A strategy to enhance doxorubicin antitumor activity. Molecular Cancer Therapeutics 3: 1167-1175 [Abstract] [Full Text]
Tetlow, L C, Woolley, D E (2003). Histamine stimulates the proliferation of human articular chondrocytes in vitro and is expressed by chondrocytes in osteoarthritic cartilage. Ann Rheum Dis 62: 991-994 [Abstract] [Full Text]
Iocca, H. A., Isom, H. C. (2003). Tumor Necrosis Factor-{alpha} Acts as a Complete Mitogen for Primary Rat Hepatocytes. Am. J. Pathol. 163: 465-476 [Abstract] [Full Text]
Collins, R. A., Grounds, M. D. (2001). The Role of Tumor Necrosis Factor-alpha (TNF-{alpha}) in Skeletal Muscle Regeneration: Studies in TNF-{alpha}(-/-) and TNF-{alpha}(-/-)/LT-{alpha}(-/-) Mice. J. Histochem. Cytochem. 49: 989-1002 [Abstract] [Full Text]
Foltyn, V N, Golan, T D (2001). In vitro ultraviolet irradiation induces pro-inflammatory responses in cells from premorbid SLE mice. Lupus 10: 272-283 [Abstract]
Williams, R. O, Feldmann, M., Maini, R. N (2000). Cartilage destruction and bone erosion in arthritis: the role of tumour necrosis factor alpha. Ann Rheum Dis 59: i75-80 [Full Text]
Solomon, A., Li, D.-Q., Lee, S.-B., Tseng, S. C. G. (2000). Regulation of Collagenase, Stromelysin, and Urokinase-Type Plasminogen Activator in Primary Pterygium Body Fibroblasts by Inflammatory Cytokines. IOVS 41: 2154-2163 [Abstract] [Full Text]
Levi-Schaffer, F., Garbuzenko, E., Rubin, A., Reich, R., Pickholz, D., Gillery, P., Emonard, H., Nagler, A., Maquart, F. A. X. (1999). Human eosinophils regulate human lung- and skin-derived fibroblast properties in vitro: A role for transforming growth factor beta (TGF-beta ). Proc. Natl. Acad. Sci. USA 96: 9660-9665 [Abstract] [Full Text]
Agarwal, S., Chandra, C. S., Piesco, N. P., Langkamp, H. H., Bowen, L., Baran, C. (1998). Regulation of Periodontal Ligament Cell Functions by Interleukin-1beta. Infect. Immun. 66: 932-937 [Abstract] [Full Text]
Varela, L. M., Darcy, K. M., Ip, M. M. (1997). The Epidermal Growth Factor Receptor Is Not Required for Tumor Necrosis Factor-{alpha} Action in Normal Mammary Epithelial Cells. Endocrinology 138: 3891-3900 [Abstract] [Full Text]
Schwenger, P., Bellosta, P., Vietor, I., Basilico, C., Skolnik, E. Y. (1997). Sodium salicylate induces apoptosis via p38 mitogen-activated protein kinase but inhibits tumor necrosis factor-induced c-Jun N-terminal kinase/stress-activated protein kinase�activation. Proc. Natl. Acad. Sci. USA 94: 2869-2873 [Abstract] [Full Text]
Kalb, A., Bluethmann, H., Moore, M. W., Lesslauer, W. (1996). Tumor Necrosis Factor Receptors (Tnfr) in Mouse Fibroblasts Deficient in Tnfr1 or Tnfr2 are Signaling Competent and Activate the Mitogen-activated Protein Kinase Pathway with Differential Kinetics. J. Biol. Chem. 271: 28097-28104 [Abstract] [Full Text]
Mackay, F., Majeau, G. R., Hochman, P. S., Browning, J. L. (1996). Lymphotoxin beta Receptor Triggering Induces Activation of the Nuclear Factor kappa B Transcription Factor in Some Cell Types. J. Biol. Chem. 271: 24934-24938 [Abstract] [Full Text]
Gardner, A. M., Johnson, G. L. (1996). Fibroblast Growth Factor-2 Suppression of Tumor Necrosis Factor alpha -Mediated Apoptosis Requires Ras and the Activation of Mitogen-activated Protein Kinase. J. Biol. Chem. 271: 14560-14566 [Abstract] [Full Text]
Schwenger, P., Skolnik, E. Y., Vilcek, J. (1996). Inhibition of Tumor Necrosis Factor-induced p42/p44 Mitogen-Activated Protein Kinase Activation by Sodium Salicylate. J. Biol. Chem. 271: 8089-8094 [Abstract] [Full Text]
Muthukkumar, S., Sells, S. F., Crist, S. A., Rangnekar, V. M. (1996). Interleukin-1 Induces Growth Arrest by Hypophosphorylation of the Retinoblastoma Susceptibility Gene Product RB. J. Biol. Chem. 271: 5733-5740 [Abstract] [Full Text]
Guo, D., Donner, D. B. (1996). Tumor Necrosis Factor Promotes Phosphorylation and Binding of Insulin Receptor Substrate 1 to Phosphatidylinositol 3-Kinase in 3T3-L1 Adipocytes. J. Biol. Chem. 271: 615-618 [Abstract] [Full Text]
Tanaka, H., Sukhova, G., Schwartz, D., Libby, P. (1996). Proliferating Arterial Smooth Muscle Cells After Balloon Injury Express TNF-{alpha} but Not Interleukin-1 or Basic Fibroblast Growth Factor. Arterioscler. Thromb. Vasc. Bio. 16: 12-18 [Abstract] [Full Text]
Arroyo, C. M, Von Tersch, R. L, Broomfield, C. A (1995). Activation of alpha-human tumour necrosis factor (TNF-{alpha}) by human monocytes (THP-1) exposed to 2-chloroethyl ethyl sulphide (H-MG). Hum Exp Toxicol 14: 547-553 [Abstract]
Lo, Y. Y. C., Cruz, T. F. (1995). Involvement of Reactive Oxygen Species in Cytokine and Growth Factor Induction of c-fos Expression in Chondrocytes. J. Biol. Chem. 270: 11727-11730 [Abstract] [Full Text]
Scher, R. L., Koch, W. M., Richtsmeier, W. J. (1993). Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma. Arch Otolaryngol Head Neck Surg 119: 432-438 [Abstract]
Cheng, J, Turksen, K, Yu, Q C, Schreiber, H, Teng, M, Fuchs, E (1992). Cachexia and graft-vs.-host-disease-type skin changes in keratin promoter-driven TNF alpha transgenic mice.. Genes Dev. 6: 1444-1456 [Abstract]
Ford, H. R., Hoffman, R. A., Wing, E. J., Magee, D. M., McIntyre, L., Simmons, R. L. (1989). Characterization of Wound Cytokines in the Sponge Matrix Model. Arch Surg 124: 1422-1428 [Abstract]
Meyer, J. D., Yurt, R. W., Duhaney, R., Hesse, D. G., Tracey, K. J., Fong, Y., Verma, M., Shires, G. T., Dineen, P., Lowry, S. F., Davis, J. M. (1988). Tumor Necrosis Factor--Enhanced Leukotriene B4 Generation and Chemotaxis in Human Neutrophils. Arch Surg 123: 1454-1458 [Abstract]
Richtsmeier, W. J. (1988). Interferon Gamma Induced Oncolysis: An Effect on Head and Neck Squamous Carcinoma Cultures. Arch Otolaryngol Head Neck Surg 114: 432-437 [Abstract]
Kehrl, J., Alvarez-Mon, M, Delsing, G., Fauci, A. (1987). Lymphotoxin is an important T cell-derived growth factor for human B cells. Science 238: 1144-1146 [Abstract]
Sehgal, P., May, L., Tamm, I, Vilcek, J (1987). Human beta 2 interferon and B-cell differentiation factor BSF-2 are identical. Science 235: 731-732
Christou, N. V., Mannick, J. A., West, M. A., Kasper, D. L. (1987). Lymphocyte-Macrophage Interactions in the Response to Surgical Infections. Arch Surg 122: 239-251 [Abstract]
Yarden, A, Kimchi, A (1986). Tumor necrosis factor reduces c-myc expression and cooperates with interferon-gamma in HeLa cells. Science 234: 1419-1421 [Abstract]
Fiers, W., Brouckaert, P., Devos, R., Fransen, L., Leroux-Roels, G., Remaut, E., Suffys, P., Tavernier, J., Van der Heyden, J., Van Roy, F. (1986). Lymphokines and Monokines in Anti-cancer Therapy. Cold Spring Harb Symp Quant Biol 51: 587-595 [Abstract]
Goeddel, D.V., Aggarwal, B.B., Gray, P.W., Leung, D.W., Nedwin, G.E., Palladino, M.A., Patton, J.S., Pennica, D., Shepard, H.M., Sugarman, B.J., Wong, G.H.W. (1986). Tumor Necrosis Factors: Gene Structure and Biological Activities. Cold Spring Harb Symp Quant Biol 51: 597-609 [Abstract]
Takeuchi, K., Shibamoto, S., Nagamine, K., Shigemori, I., Omura, S., Kitamura, N., Ito, F. (2001). Signaling Pathways Leading to Transcription and Translation Cooperatively Regulate the Transient Increase in Expression of c-Fos Protein. J. Biol. Chem. 276: 26077-26083 [Abstract] [Full Text]