Journal of Experimental Medicine, Vol 162, 1099-1104, Copyright © 1985 by Rockefeller University Press

ARTICLES

High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors

BY Rubin, SL Anderson, SA Sullivan, BD Williamson, EA Carswell and LJ Old

125I-labeled TNF(LuKII) (tumor necrosis factor) binds specifically to human and mouse cell lines sensitive to the cytotoxic effect of TNF, but not to cells made resistant to TNF. TNF-sensitive cells have cell surface receptors with a high affinity for TNF(LuKII). Mouse TNF competes with TNF(LuKII) for receptor binding. Scatchard analysis of the binding data yielded linear plots and suggests that TNF(LuKII) binds to homogeneous receptor sites. The number of TNF(LuKII) receptors on two TNF-sensitive cell lines is 200-300 per cell and the affinity constant of the receptor for TNF(LuKII) is approximately 1 X 10(-10) M.
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