Location of variable and conserved epitopes among the multiple serotypes of streptococcal M protein

doi:10.1084/jem.161.3.623

This Article

	Full Text (PDF, 466K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Jones, K. F.
	Articles by Fischetti, V. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Jones, K. F.
	Articles by Fischetti, V. A.


Social Bookmarking

	What's this?

ARTICLES

Location of variable and conserved epitopes among the multiple serotypes of streptococcal M protein

KF Jones, BN Manjula, KH Johnston, SK Hollingshead, JR Scott and VA Fischetti

In studies primarily confined to the amino-terminal region of the fibrillar group A streptococcal M protein, only limited immunological crossreactions have been observed among M serotypes. In this investigation, two monoclonal antibodies generated against nearly the entire M6 molecule (LysM6) were used to determine the extent of crossreactions among M serotyping strains and to localize their epitopes on the M molecule. Colony blot and immunoblot analyses revealed that an epitope responsible for crossreactions among 5 of the 56 strains of different M serotypes tested is located in the amino- terminal half of the molecule, distal to the cell surface. In contrast, a more common crossreactive epitope, reacting with 20 of the 56 strains, is located near the middle of the M molecule. These studies also reveal that the more conserved determinant, located more proximally to the cell surface, is accessible to the immune system, even on the whole organism, and, thus, may be useful in devising means to protect against infections by multiple group A streptococcal M serotypes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Luca-Harari, B., Darenberg, J., Neal, S., Siljander, T., Strakova, L., Tanna, A., Creti, R., Ekelund, K., Koliou, M., Tassios, P. T., van der Linden, M., Straut, M., Vuopio-Varkila, J., Bouvet, A., Efstratiou, A., Schalen, C., Henriques-Normark, B., the Strep-EURO Study Group, , Jasir, A. (2009). Clinical and Microbiological Characteristics of Severe Streptococcus pyogenes Disease in Europe. J. Clin. Microbiol. 47: 1155-1165 [Abstract] [Full Text]
Raz, A., Fischetti, V. A. (2008). Sortase A localizes to distinct foci on the Streptococcus pyogenes membrane. Proc. Natl. Acad. Sci. USA 105: 18549-18554 [Abstract] [Full Text]
Ellis, N. M. J., Li, Y., Hildebrand, W., Fischetti, V. A., Cunningham, M. W. (2005). T Cell Mimicry and Epitope Specificity of Cross-Reactive T Cell Clones from Rheumatic Heart Disease. J. Immunol. 175: 5448-5456 [Abstract] [Full Text]
Boel, G., Jin, H., Pancholi, V. (2005). Inhibition of Cell Surface Export of Group A Streptococcal Anchorless Surface Dehydrogenase Affects Bacterial Adherence and Antiphagocytic Properties. Infect. Immun. 73: 6237-6248 [Abstract] [Full Text]
Barnett, T. C., Patel, A. R., Scott, J. R. (2004). A Novel Sortase, SrtC2, from Streptococcus pyogenes Anchors a Surface Protein Containing a QVPTGV Motif to the Cell Wall. J. Bacteriol. 186: 5865-5875 [Abstract] [Full Text]
McGregor, K. F., Spratt, B. G., Kalia, A., Bennett, A., Bilek, N., Beall, B., Bessen, D. E. (2004). Multilocus Sequence Typing of Streptococcus pyogenes Representing Most Known emm Types and Distinctions among Subpopulation Genetic Structures. J. Bacteriol. 186: 4285-4294 [Abstract] [Full Text]
Mannam, P., Jones, K. F., Geller, B. L. (2004). Mucosal Vaccine Made from Live, Recombinant Lactococcus lactis Protects Mice against Pharyngeal Infection with Streptococcus pyogenes. Infect. Immun. 72: 3444-3450 [Abstract] [Full Text]
Towers, R. J., Fagan, P. K., Talay, S. R., Currie, B. J., Sriprakash, K. S., Walker, M. J., Chhatwal, G. S. (2003). Evolution of sfbI Encoding Streptococcal Fibronectin-Binding Protein I: Horizontal Genetic Transfer and Gene Mosaic Structure. J. Clin. Microbiol. 41: 5398-5406 [Abstract] [Full Text]
Hu, M. C., Walls, M. A., Stroop, S. D., Reddish, M. A., Beall, B., Dale, J. B. (2002). Immunogenicity of a 26-Valent Group A Streptococcal Vaccine. Infect. Immun. 70: 2171-2177 [Abstract] [Full Text]
Ryan, P. A., Pancholi, V., Fischetti, V. A. (2001). Group A Streptococci Bind to Mucin and Human Pharyngeal Cells through Sialic Acid-Containing Receptors. Infect. Immun. 69: 7402-7412 [Abstract] [Full Text]
Manna, A., Cheung, A. L. (2001). Characterization of sarR, a Modulator of sar Expression in Staphylococcus aureus. Infect. Immun. 69: 885-896 [Abstract] [Full Text]
Cheung, A. L., Chien, Y.-t., Bayer, A. S. (1999). Hyperproduction of Alpha-Hemolysin in a sigB Mutant Is Associated with Elevated SarA Expression in Staphylococcus aureus. Infect. Immun. 67: 1331-1337 [Abstract] [Full Text]
Berneman, A., Belec, L., Fischetti, V. A., Bouvet, J.-P. (1998). The Specificity Patterns of Human Immunoglobulin G Antibodies in Serum Differ from Those in Autologous Secretions. Infect. Immun. 66: 4163-4168 [Abstract] [Full Text]
Blackmore, T. K., Fischetti, V. A., Sadlon, T. A., Ward, H. M., Gordon, D. L. (1998). M Protein of the Group A Streptococcus Binds to the Seventh Short Consensus Repeat of Human Complement Factor H. Infect. Immun. 66: 1427-1431 [Abstract] [Full Text]
Fischetti, V., Hodges, W., Hruby, D. (1989). Protection against streptococcal pharyngeal colonization with a vaccinia: M protein recombinant. Science 244: 1487-1490 [Abstract]