Journal of Experimental Medicine, Vol 160, 1919-1924, Copyright © 1984 by Rockefeller University Press
Selective inhibition of growth factor-dependent human B cell proliferation by monoclonal antibody AB1 to an antigen expressed by activated B cells
LK Jung and SM Fu
A monoclonal antibody, AB1, was established with activated human B cells as
immunogen. AB1 stained activated B cells but not activated T cells. Its
selective reactivity to activated B cells was further documented by its
nonreactivity to activated T cells, resting T and B cells, monocytes,
granulocytes, bone marrow cells, leukemic cells, and cells from cell lines
of T, B, and myeloid lineages. Upon activation, the antigen appeared on B
cells as early as 3-4 h after stimulation and was fully expressed by 38 h.
The expression of this antigen was not dependent on the presence of B cell
stimulatory factor(s). Anti-IgM antibodies by themselves induced its
expression. AB1 inhibited B cell proliferation that was induced by a low
dose anti-IgM antibody and conditioned medium containing B cell stimulatory
factor. It did not inhibit B cell proliferation induced by either high
doses of anti-IgM antibodies or by formalinized Staphylococcus aureus. It
also failed to inhibit T cell mitogenesis. The possibility exists that this
antigen is related to the receptor for B cell stimulatory factor.
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