Both interleukin 2 and a second T cell-derived factor in EL-4 supernatant have activity as differentiation factors in IgM synthesis

doi:10.1084/jem.160.6.1605

This Article

	Full Text (PDF, 1110K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Nakanishi, K.
	Articles by Paul, W. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Nakanishi, K.
	Articles by Paul, W. E.


Social Bookmarking

	What's this?

ARTICLES

Both interleukin 2 and a second T cell-derived factor in EL-4 supernatant have activity as differentiation factors in IgM synthesis

K Nakanishi, TR Malek, KA Smith, T Hamaoka, EM Shevach and WE Paul

B cells cultured with anti-IgM, BSF-p1, and B15-TRF will differentiate into high rate IgM-synthesizing cells in the presence of supernatants from EL-4 cells that have been induced with phorbol myristate acetate. These supernatants contain two molecular species (EL-TRFs) that have differentiative activity. One co-migrates with interleukin 2 (IL-2) and its activity is blocked by antibody to the IL-2 receptor. Furthermore, molecularly cloned IL-2, at concentrations of 100 U/ml or more, expresses such EL-TRF activity. The EL-TRF activity of cloned IL-2 can also be inhibited by antibody to the IL-2 receptor. The other material with EL-TRF activity has a molecular weight of approximately 32,000. This material lacks IL-2 activity. Antibody to the IL-2 receptor does not impair its function. B cells stimulated with anti-IgM and BSF-p1, with or without B15-TRF, express determinants that react with two monoclonal antibodies which recognize distinct epitopes on the T cell IL-2 receptor. These determinants are present at much lower density (approximately 100-fold) on stimulated B cells that on HT-2 cells, an IL-2-dependent T cell line. Very small amounts of [3H]IL-2 (less than 1,000 molecules per cell) bind to activated B cells. These results indicate that IL-2 binds to a receptor on appropriately prepared B cells and causes them to differentiate into high rate IgM-synthesizing cells. The physiologic significance of the B cell differentiative activity of IL-2 remains to be investigated.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Appay, V., Zaunders, J. J., Papagno, L., Sutton, J., Jaramillo, A., Waters, A., Easterbrook, P., Grey, P., Smith, D., McMichael, A. J., Cooper, D. A., Rowland-Jones, S. L., Kelleher, A. D. (2002). Characterization of CD4+ CTLs Ex Vivo. J. Immunol. 168: 5954-5958 [Abstract] [Full Text]
Yoshimoto, T., Okamura, H., Tagawa, Y.-I., Iwakura, Y., Nakanishi, K. (1997). Interleukin 18�together with interleukin 12�inhibits IgE production by induction of interferon-gamma production from activated B�cells. Proc. Natl. Acad. Sci. USA 94: 3948-3953 [Abstract] [Full Text]
Eardley, D., Koshland, M. (1991). Glycosylphosphatidylinositol: a candidate system for interleukin-2 signal transduction. Science 251: 78-81 [Abstract]
Tigges, M., Casey, L., Koshland, M. (1989). Mechanism of interleukin-2 signaling: mediation of different outcomes by a single receptor and transduction pathway. Science 243: 781-786 [Abstract]
Smith, K. (1988). Interleukin-2: inception, impact, and implications. Science 240: 1169-1176 [Abstract]
Wood, J. J., O'Mahony, J. B., Rodrick, M. L., Eaton, R., Demling, R. H., Mannick, J. A. (1986). Abnormalities of Antibody Production After Thermal Injury: An Association With Reduced Interleukin 2 Production. Arch Surg 121: 108-115 [Abstract]