Journal of Experimental Medicine, Vol 160, 323-328, Copyright © 1984 by Rockefeller University Press

ARTICLES

Isolation, characterization, and propagation in vitro of human glomerular endothelial cells

GE Striker, C Soderland, DF Bowen-Pope, AM Gown, G Schmer, A Johnson, D Luchtel, R Ross and LJ Striker

Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE).
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Ray, P. E., Tassi, E., Liu, X.-H., Wellstein, A. (2006). Role of fibroblast growth factor-binding protein in the pathogenesis of HIV-associated hemolytic uremic syndrome. Am. J. Physiol. Regul. Integr. Comp. Physiol. 290: R105-R113 [Abstract] [Full Text]  
• Scott, P., Bicknell, R (1993). The isolation and culture of microvascular endothelium. J. Cell Sci. 105: 269-273  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS