Journal of Experimental Medicine, Vol 160, 167-178, Copyright © 1984 by Rockefeller University Press

ARTICLES

Dissection of serological and cytolytic T lymphocyte epitopes on murine major histocompatibility antigens by a recombinant H-2 gene separating the first two external domains

C Murre, E Choi, J Weis, JG Seidman, K Ozato, L Liu, SJ Burakoff and CS Reiss

A novel H-2 gene in which the first external (N) domain of the H-2Ld antigen was replaced with that of the H-2Dd antigen was constructed and introduced into L cells. A transformant expressing the products of the hybrid gene was studied for binding to monoclonal antibodies specific for H-2Ld and H-2Dd antigens. It was found that serological determinants are distributed both in the N (Dd) and Cl (Ld) domains. Determinants recognized by allospecific cytotoxic T lymphocytes (CTLs) and virus-specific CTLs also mapped to the N and Cl domains. Determinants recognized by vesicular stomatitis virus (VSV)-specific effect cells, however, were not present on the recombinant molecule. These results show that a recombinant gene of two H-2 antigens in which the first external domain has been reshuffled can express a functional H-2 antigen that can then be used to map serological and CTL determinants to specific domains.
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This article has been cited by other articles:

• Smith, R. A., Myers, N. B., Robinson, M., Hansen, T. H., Lee, D. R. (2002). Polymorphism at Position 97 in MHC Class I Molecules Affects Peptide Specificity, Cell Surface Stability, and Affinity for {beta}2-Microglobulin. J. Immunol. 169: 3105-3111 [Abstract] [Full Text]  
• Flavell, R., Allen, H, Burkly, L., Sherman, D., Waneck, G., Widera, G (1986). Molecular biology of the H-2 histocompatibility complex. Science 233: 437-443 [Abstract]  

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