Gut mucosal mast cells. Origin, traffic, and differentiation

doi:10.1084/jem.160.1.12

This Article

	Full Text (PDF, 2066K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Guy-Grand, D.
	Articles by Vassalli, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Guy-Grand, D.
	Articles by Vassalli, P.


Social Bookmarking

	What's this?

ARTICLES

Gut mucosal mast cells. Origin, traffic, and differentiation

D Guy-Grand, M Dy, G Luffau and P Vassalli

Gut mucosal mast cells (MMC), which are nearly absent in normal mice are abundant during nematode infection. In normal mice, study of MMC precursors (MMC-P: cells giving rise to MMC colonies in the presence of IL-3) show that: (a) their frequency, judged by limiting dilution is very high in bone marrow (BM) and gut, and very low in most lymphoid organs and thoracic duct lymph (TDL); (b) gut MMC-P are Thy-1- Lyt-1-2- and are not rapidly replicating; (c) they are the progeny of less differentiated BM MMC-P which are attracted from the blood to the gut mucosa by local factor(s), other than antigen and T cell factors (since normal amounts of gut MMC-P are found in germ-free, nude, and newborn mice). In mice bearing the Wehi 3 tumor (which releases enough IL-3 to produce detectable blood levels) spleen and mesenteric lymph nodes (LN) show increased MMC-P frequency, the greatest increase being in the gut and BM, where numerous differentiated MMC are found. In Nippostrongylus brasiliensis (Nb)-infested mice (known to develop a large, T cell- dependent, gut MMC infiltration), gut MMC-P proliferation is induced by IL-3 released from gut mucosal Thy-1+ Lyt-2- cells, whose in vitro IL-3 release capability is much higher than that of similar cells from normal mice. Both Nb-stimulated T blasts and proliferating MMC-P undergo cyclic traffic, migrating into the TDL and then seeding the whole length of the gut (a process which allows a widespread immune defense after a local antigenic stimulus). Experiments using 2-d interruption of this traffic and fetal gut grafts, suggest that the continuous homing of T blasts back to the gut which leads to permanent Nb-stimulated IL-3 release, is essential for the full maturation of MMC. Transfer experiments in the rat show that TDL circulating MMC-P rapidly mature into MMC when they home back to the Nb-infested gut. It is proposed that gut MMC arise after several stages of progressive differentiation of MMC-P, influenced both by IL-3 and unidentified gut factor(s).
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Jones, T. G., Hallgren, J., Humbles, A., Burwell, T., Finkelman, F. D., Alcaide, P., Austen, K. F., Gurish, M. F. (2009). Antigen-Induced Increases in Pulmonary Mast Cell Progenitor Numbers Depend on IL-9 and CD1d-Restricted NKT Cells. J. Immunol. 183: 5251-5260 [Abstract] [Full Text]
Wang, D., Ma, W., She, R., Sun, Qu., Liu, Y., Hu, Y., Liu, L., Yang, Y., Peng, K. (2009). Effects of swine gut antimicrobial peptides on the intestinal mucosal immunity in specific-pathogen-free chickens. Poult. Sci. 88: 967-974 [Abstract] [Full Text]
Hallgren, J., Jones, T. G., Abonia, J. P., Xing, W., Humbles, A., Austen, K. F., Gurish, M. F. (2007). Pulmonary CXCR2 regulates VCAM-1 and antigen-induced recruitment of mast cell progenitors. Proc. Natl. Acad. Sci. USA 104: 20478-20483 [Abstract] [Full Text]
Alcaide, P., Jones, T. G., Lord, G. M., Glimcher, L. H., Hallgren, J., Arinobu, Y., Akashi, K., Paterson, A. M., Gurish, M. A., Luscinskas, F. W. (2007). Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue. JEM 204: 431-439 [Abstract] [Full Text]
Abonia, J. P., Hallgren, J., Jones, T., Shi, T., Xu, Y., Koni, P., Flavell, R. A., Boyce, J. A., Austen, K. F., Gurish, M. F. (2006). Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung. Blood 108: 1588-1594 [Abstract] [Full Text]
Abonia, J. P., Austen, K. F., Rollins, B. J., Joshi, S. K., Flavell, R. A., Kuziel, W. A., Koni, P. A., Gurish, M. F. (2005). Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2. Blood 105: 4308-4313 [Abstract] [Full Text]
Gurish, M. F., Humbles, A., Tao, H., Finkelstein, S., Boyce, J. A., Gerard, C., Friend, D. S., Austen, K. F. (2002). CCR3 Is Required for Tissue Eosinophilia and Larval Cytotoxicity After Infection with Trichinella spiralis. J. Immunol. 168: 5730-5736 [Abstract] [Full Text]
Boyce, J. A., Mellor, E. A., Perkins, B., Lim, Y.-C., Luscinskas, F. W. (2002). Human mast cell progenitors use alpha 4-integrin, VCAM-1, and PSGL-1 E-selectin for adhesive interactions with human vascular endothelium under flow conditions. Blood 99: 2890-2896 [Abstract] [Full Text]
Lambolez, F., Azogui, O., Joret, A.-M., Garcia, C., von Boehmer, H., Di Santo, J., Ezine, S., Rocha, B. (2002). Characterization of T Cell Differentiation in the Murine Gut. JEM 195: 437-449 [Abstract] [Full Text]
Gurish, M. F., Tao, H., Abonia, J. P., Arya, A., Friend, D. S., Parker, C. M., Austen, K. F. (2001). Intestinal Mast Cell Progenitors Require CD49d{beta}7 ({alpha}4{beta}7 Integrin) for Tissue-specific Homing. JEM 194: 1243-1252 [Abstract] [Full Text]
Gurish, M. F., Austen, K. F. (2001). The Diverse Roles of Mast Cells. JEM 194: f1-f6 [Full Text]
Yuan, Q., Gurish, M. F., Friend, D. S., Austen, K. F., Boyce, J. A. (1998). Cutting Edge: Generation of a Novel Stem Cell Factor-Dependent Mast Cell Progenitor. J. Immunol. 161: 5143-5146 [Abstract] [Full Text]