Journal of Experimental Medicine, Vol 159, 479-494, Copyright © 1984 by Rockefeller University Press

ARTICLES

Activation specificity of arsonate-reactive T cell clones. Structural requirements for hapten recognition and comparison with monoclonal antibodies

A Rao, SJ Faas and H Cantor

We describe clones of hapten-specific inducer T cells from (BALB/c X A/J)F1 mice that respond to the p-azobenzenearsonate hapten conjugated to carrier proteins or directly conjugated to antigen-presenting cells. Some of the clones are also activated by haptens structurally related to arsonate. All activating analogues are recognized by each clone in association with the same major histocompatibility complex (MHC) protein as is arsonate. Weakly activating and nonactivating analogues are immunogenic in D2.GD amd (BALB/c X A/J)F1 mice, since they can effectively activate primed lymph node cells or long-term hapten- reactive cell lines. Hence the specificities of these clones may reflect their intrinsic recognition of arsonate and its analogues, rather than more efficient presentation of certain analogues than of others by antigen-presenting cells, or differential recognition of associated MHC epitopes by the clones. We compare the activation specificities of the clones with the binding specificities of monoclonal antibodies to arsonate, and discuss structural features of the analogues that may be important for activation and binding. Our results suggest that a site (or subsite) on arsonate-reactive T cell clones may interact directly with hapten, and may be experimentally separable from the site (or subsite) for MHC determinants.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Shimizu, T., Osaka, Y., Banri-Koike, C., Yoshida, M., Endo, K., Furukawa, K., Oda, M., Murakami, A., Ogawa, S., Abe, R., Azuma, T. (2007). T cells specific to hapten carrier but not to carrier alone assist in the production of anti-hapten and anti-carrier antibodies. Int Immunol 19: 1157-1164 [Abstract] [Full Text]  
• Herzog, J., Maekawa, Y., Cirrito, T. P., Illian, B. S., Unanue, E. R. (2005). Activated antigen-presenting cells select and present chemically modified peptides recognized by unique CD4 T cells. Proc. Natl. Acad. Sci. USA 102: 7928-7933 [Abstract] [Full Text]  
• Im, S.-H., Hueber, A., Monticelli, S., Kang, K.-H., Rao, A. (2004). Chromatin-level Regulation of the IL10 Gene in T Cells. J. Biol. Chem. 279: 46818-46825 [Abstract] [Full Text]  
• Okamura, H., Garcia-Rodriguez, C., Martinson, H., Qin, J., Virshup, D. M., Rao, A. (2004). A Conserved Docking Motif for CK1 Binding Controls the Nuclear Localization of NFAT1. Mol. Cell. Biol. 24: 4184-4195 [Abstract] [Full Text]  
• Loh, C., Shaw, K. T.-Y., Carew, J., Viola, J. P. B., Luo, C., Perrino, B. A., Rao, A. (1996). Calcineurin Binds the Transcription Factor NFAT1 and Reversibly Regulates Its Activity. J. Biol. Chem. 271: 10884-10891 [Abstract] [Full Text]  
• Nalefski, E. A., Shaw, K. T. Y., Rao, A. (1995). An Ion Pair in Class II Major Histocompatibility Complex Heterodimers Critical for Surface Expression and Peptide Presentation. J. Biol. Chem. 270: 22351-22360 [Abstract] [Full Text]  
• McCaffrey, P., Luo, C, Kerppola, T., Jain, J, Badalian, T., Ho, A., Burgeon, E, Lane, W., Lambert, J., Curran, T, et, al. (1993). Isolation of the cyclosporin-sensitive T cell transcription factor NFATp. Science 262: 750-754 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS