Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes

doi:10.1084/jem.158.6.2016

This Article

	Full Text (PDF, 574K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wright, S. D.
	Articles by Silverstein, S. C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wright, S. D.
	Articles by Silverstein, S. C.


Social Bookmarking

	What's this?

ARTICLES

Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes

SD Wright and SC Silverstein

We have measured the release of H2O2 from granulocytes, monocytes, and macrophages during spreading on ligand-coated culture surfaces. While IgG-coated surfaces stimulate vigorous release of H2O2, neither C3b- nor C3bi-coated surfaces promoted appreciable release of H2O2 despite full ligation of C3b and C3bi receptors. We also measured release of H2O2 from cultured monocytes spreading on surfaces coated with both fibronectin and C3. Under such circumstances, the C3 receptors elicit a strong phagocytic response, but no H2O2 release was recorded. We conclude that the C3b and C3bi receptors of monocytes and granulocytes do not signal the generation of toxic oxygen intermediates from these cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Schulert, G. S., McCaffrey, R. L., Buchan, B. W., Lindemann, S. R., Hollenback, C., Jones, B. D., Allen, L.-A. H. (2009). Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS. Infect. Immun. 77: 1324-1336 [Abstract] [Full Text]
N'Diaye, E.-N., Branda, C. S., Branda, S. S., Nevarez, L., Colonna, M., Lowell, C., Hamerman, J. A., Seaman, W. E. (2009). TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria. JCB 184: 215-223 [Abstract] [Full Text]
Kemper, C., Mitchell, L. M., Zhang, L., Hourcade, D. E. (2008). The complement protein properdin binds apoptotic T cells and promotes complement activation and phagocytosis. Proc. Natl. Acad. Sci. USA 105: 9023-9028 [Abstract] [Full Text]
Wang, M., Shakhatreh, M.-A. K., James, D., Liang, S., Nishiyama, S.-i., Yoshimura, F., Demuth, D. R., Hajishengallis, G. (2007). Fimbrial Proteins of Porphyromonas gingivalis Mediate In Vivo Virulence and Exploit TLR2 and Complement Receptor 3 to Persist in Macrophages. J. Immunol. 179: 2349-2358 [Abstract] [Full Text]
Hajishengallis, G., Shakhatreh, M.-A. K., Wang, M., Liang, S. (2007). Complement Receptor 3 Blockade Promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. J. Immunol. 179: 2359-2367 [Abstract] [Full Text]
Chang, H. K., Thalhofer, C., Duerkop, B. A., Mehling, J. S., Verma, S., Gollob, K. J., Almeida, R., Wilson, M. E. (2007). Oxidant Generation by Single Infected Monocytes after Short-Term Fluorescence Labeling of a Protozoan Parasite. Infect. Immun. 75: 1017-1024 [Abstract] [Full Text]
Lavigne, L. M., Albina, J. E., Reichner, J. S. (2006). beta-Glucan Is a Fungal Determinant for Adhesion-Dependent Human Neutrophil Functions. J. Immunol. 177: 8667-8675 [Abstract] [Full Text]
Li, Z., Burns, A. R., Smith, C. W. (2006). Lymphocyte Function-Associated Antigen-1-Dependent Inhibition of Corneal Wound Healing. Am. J. Pathol. 169: 1590-1600 [Abstract] [Full Text]
Hajishengallis, G., Wang, M., Harokopakis, E., Triantafilou, M., Triantafilou, K. (2006). Porphyromonas gingivalis Fimbriae Proactively Modulate {beta}2 Integrin Adhesive Activity and Promote Binding to and Internalization by Macrophages.. Infect. Immun. 74: 5658-5666 [Abstract] [Full Text]
Clemens, D. L., Lee, B.-Y., Horwitz, M. A. (2005). Francisella tularensis Enters Macrophages via a Novel Process Involving Pseudopod Loops. Infect. Immun. 73: 5892-5902 [Abstract] [Full Text]
Brandhorst, T. T., Wuthrich, M., Finkel-Jimenez, B., Warner, T., Klein, B. S. (2004). Exploiting Type 3 Complement Receptor for TNF-{alpha} Suppression, Immune Evasion, and Progressive Pulmonary Fungal Infection. J. Immunol. 173: 7444-7453 [Abstract] [Full Text]
Edwards, J. L., Apicella, M. A. (2004). The Molecular Mechanisms Used by Neisseria gonorrhoeae To Initiate Infection Differ between Men and Women. Clin. Microbiol. Rev. 17: 965-981 [Abstract] [Full Text]
Stokes, R. W., Norris-Jones, R., Brooks, D. E., Beveridge, T. J., Doxsee, D., Thorson, L. M. (2004). The Glycan-Rich Outer Layer of the Cell Wall of Mycobacterium tuberculosis Acts as an Antiphagocytic Capsule Limiting the Association of the Bacterium with Macrophages. Infect. Immun. 72: 5676-5686 [Abstract] [Full Text]
Townsend, S. M., Pollack, H. A., Gonzalez-Gomez, I., Shimada, H., Badger, J. L. (2003). Citrobacter koseri Brain Abscess in the Neonatal Rat: Survival and Replication within Human and Rat Macrophages. Infect. Immun. 71: 5871-5880 [Abstract] [Full Text]
Li, Y., Zhang, L. (2003). The Fourth Blade within the {beta}-Propeller Is Involved Specifically in C3bi Recognition by Integrin {alpha}M{beta}2. J. Biol. Chem. 278: 34395-34402 [Abstract] [Full Text]
Marr, K. A., Arunmozhi Balajee, S., Hawn, T. R., Ozinsky, A., Pham, U., Akira, S., Aderem, A., Conrad Liles, W. (2003). Differential Role of MyD88 in Macrophage-Mediated Responses to Opportunistic Fungal Pathogens. Infect. Immun. 71: 5280-5286 [Abstract] [Full Text]
Indrigo, J., Hunter, R. L. Jr, Actor, J. K. (2003). Cord factor trehalose 6,6'-dimycolate (TDM) mediates trafficking events during mycobacterial infection of murine macrophages. Microbiology 149: 2049-2059 [Abstract] [Full Text]
Caragine, T. A., Imai, M., Frey, A. B., Tomlinson, S. (2002). Expression of rat complement control protein Crry on tumor cells inhibits rat natural killer cell-mediated cytotoxicity. Blood 100: 3304-3310 [Abstract] [Full Text]
Kobayashi, S. D., Voyich, J. M., Buhl, C. L., Stahl, R. M., DeLeo, F. R. (2002). Global changes in gene expression by human polymorphonuclear leukocytes during receptor-mediated phagocytosis: Cell fate is regulated at the level of gene expression. Proc. Natl. Acad. Sci. USA 99: 6901-6906 [Abstract] [Full Text]
Thieringer, R., Le Grand, C. B., Carbin, L., Cai, T.-Q., Wong, B., Wright, S. D., Hermanowski-Vosatka, A. (2001). 11{{beta}}-Hydroxysteroid Dehydrogenase Type 1 Is Induced in Human Monocytes upon Differentiation to Macrophages. J. Immunol. 167: 30-35 [Abstract] [Full Text]
van Spriel, A. B., Leusen, J. H. W., van Egmond, M., Dijkman, H. B. P. M., Assmann, K. J. M., Mayadas, T. N., van de Winkel, J. G. J. (2001). Mac-1 (CD11b/CD18) is essential for Fc receptor-mediated neutrophil cytotoxicity and immunologic synapse formation. Blood 97: 2478-2486 [Abstract] [Full Text]
Ren, Y., Stuart, L., Lindberg, F. P., Rosenkranz, A. R., Chen, Y., Mayadas, T. N., Savill, J. (2001). Nonphlogistic Clearance of Late Apoptotic Neutrophils by Macrophages: Efficient Phagocytosis Independent of {{beta}}2 Integrins. J. Immunol. 166: 4743-4750 [Abstract] [Full Text]
Bandyopadhyay, P., Steinman, H. M. (2000). Catalase-Peroxidases of Legionella pneumophila: Cloning of the katA Gene and Studies of KatA Function. J. Bacteriol. 182: 6679-6686 [Abstract] [Full Text]
Gershov, D., Kim, S., Brot, N., Elkon, K. B. (2000). C-Reactive Protein Binds to Apoptotic Cells, Protects the Cells from Assembly of the Terminal Complement Components, and Sustains an Antiinflammatory Innate Immune Response: Implications for Systemic Autoimmunity. JEM 192: 1353-1364 [Abstract] [Full Text]
Su{beta}muth, S. D., Muscholl-Silberhorn, A., Wirth, R., Susa, M., Marre, R., Rozdzinski, E. (2000). Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst. Infect. Immun. 68: 4900-4906 [Abstract] [Full Text]
Hu, C., Mayadas-Norton, T., Tanaka, K., Chan, J., Salgame, P. (2000). Mycobacterium tuberculosis Infection in Complement Receptor 3-Deficient Mice. J. Immunol. 165: 2596-2602 [Abstract] [Full Text]
Larsen, E. C., DiGennaro, J. A., Saito, N., Mehta, S., Loegering, D. J., Mazurkiewicz, J. E., Lennartz, M. R. (2000). Differential Requirement for Classic and Novel PKC Isoforms in Respiratory Burst and Phagocytosis in RAW 264.7 Cells. J. Immunol. 165: 2809-2817 [Abstract] [Full Text]
Le Cabec, V., Cols, C., Maridonneau-Parini, I. (2000). Nonopsonic Phagocytosis of Zymosan and Mycobacterium kansasii by CR3 (CD11b/CD18) Involves Distinct Molecular Determinants and Is or Is Not Coupled with NADPH Oxidase Activation. Infect. Immun. 68: 4736-4745 [Abstract] [Full Text]
Fratazzi, C., Manjunath, N., Arbeit, R. D., Carini, C., Gerken, T. A., Ardman, B., Remold-O'Donnell, E., Remold, H. G. (2000). A Macrophage Invasion Mechanism for Mycobacteria Implicating the Extracellular Domain of Cd43. JEM 192: 183-192 [Abstract] [Full Text]
Hetland, G, Namork, E, Schwarze, P E, Aase, A (2000). Mechanism for uptake of silica particles by monocytic U937 cells. Hum Exp Toxicol 19: 412-419 [Abstract]
Simon, S. I., Hu, Y., Vestweber, D., Smith, C. W. (2000). Neutrophil Tethering on E-Selectin Activates {beta}2 Integrin Binding to ICAM-1 Through a Mitogen-Activated Protein Kinase Signal Transduction Pathway. J. Immunol. 164: 4348-4358 [Abstract] [Full Text]
Malik, Z. A., Denning, G. M., Kusner, D. J. (2000). Inhibition of Ca2+ Signaling by Mycobacterium tuberculosisIs Associated with Reduced Phagosome-Lysosome Fusion and Increased Survival within Human Macrophages. JEM 191: 287-302 [Abstract] [Full Text]
Eze, M. O., Yuan, L., Crawford, R. M., Paranavitana, C. M., Hadfield, T. L., Bhattacharjee, A. K., Warren, R. L., Hoover, D. L. (2000). Effects of Opsonization and Gamma Interferon on Growth of Brucella melitensis 16M in Mouse Peritoneal Macrophages In Vitro. Infect. Immun. 68: 257-263 [Abstract] [Full Text]
Capo, C., Lindberg, F. P., Meconi, S., Zaffran, Y., Tardei, G., Brown, E. J., Raoult, D., Mege, J.-L. (1999). Subversion of Monocyte Functions by Coxiella burnetii: Impairment of the Cross-Talk Between {alpha}v{beta}3 Integrin and CR3. J. Immunol. 163: 6078-6085 [Abstract] [Full Text]
Cirillo, J. D., Cirillo, S. L. G., Yan, L., Bermudez, L. E., Falkow, S., Tompkins, L. S. (1999). Intracellular Growth in Acanthamoeba castellanii Affects Monocyte Entry Mechanisms and Enhances Virulence of Legionella pneumophila. Infect. Immun. 67: 4427-4434 [Abstract] [Full Text]
Fabian, I., Halperin, D., Lefter, S., Mittelman, L., Altstock, R. T., Seaon, O., Tsarfaty, I. (1999). Alteration of Actin Organization by Jaspamide Inhibits Ruffling, but not Phagocytosis or Oxidative Burst, in HL-60 Cells and Human Monocytes. Blood 93: 3994-4005 [Abstract] [Full Text]
Harth, G., Horwitz, M. A. (1999). Export of Recombinant Mycobacterium tuberculosis Superoxide Dismutase Is Dependent upon Both Information in the Protein and Mycobacterial Export Machinery. A MODEL FOR STUDYING EXPORT OF LEADERLESS PROTEINS BY PATHOGENIC MYCOBACTERIA. J. Biol. Chem. 274: 4281-4292 [Abstract] [Full Text]
Astarie-Dequeker, C., N'Diaye, E.-N., Le Cabec, V., Rittig, M. G., Prandi, J., Maridonneau-Parini, I. (1999). The Mannose Receptor Mediates Uptake of Pathogenic and Nonpathogenic Mycobacteria and Bypasses Bactericidal Responses in Human Macrophages. Infect. Immun. 67: 469-477 [Abstract] [Full Text]
Dominguez, M., Torano, A. (1999). Immune Adherence-mediated Opsonophagocytosis: The Mechanism of Leishmania Infection. JEM 189: 25-35 [Abstract] [Full Text]
Mevorach, D., Mascarenhas, J. O., Gershov, D., Elkon, K. B. (1998). Complement-dependent Clearance of Apoptotic Cells by Human Macrophages. JEM 188: 2313-2320 [Abstract] [Full Text]
Caron, E., Hall, A. (1998). Identification of Two Distinct Mechanisms of Phagocytosis Controlled by Different Rho GTPases. Science 282: 1717-1721 [Abstract] [Full Text]
Abu Kwaik, Y., Venkataraman, C., Harb, O. S., Gao, L.-Y. (1998). Signal Transduction in the Protozoan Host Hartmannella vermiformis upon Attachment and Invasion by Legionella micdadei. Appl. Environ. Microbiol. 64: 3134-3139 [Abstract] [Full Text]
Ehrt, S., Shiloh, M. U., Ruan, J., Choi, M., Gunzburg, S., Nathan, C., Xie, Q.-w., Riley, L. W. (1997). A Novel Antioxidant Gene from Mycobacterium tuberculosis. JEM 186: 1885-1896 [Abstract] [Full Text]
Van Nhieu, G. T., Krukonis, E. S., Reszka, A. A., Horwitz, A. F., Isberg, R. R. (1996). Mutations in the Cytoplasmic Domain of the Integrin beta(1) Chain Indicate a Role for Endocytosis Factors in Bacterial Internalization. J. Biol. Chem. 271: 7665-7672 [Abstract] [Full Text]
Crawford, J. M., Watanabe, K. (1994). Cell Adhesion Molecules in Inflammation and Immunity: Relevance to Periodontal Diseases. CROBM 5: 91-123 [Abstract] [Full Text]
Garcia-Aguilar, J., Lanser, M. E., Brown, G. E. (1988). Coagulation Augments Neutrophil C3b Receptors via Formation of a Protein(s) Unrelated to Fibrinolysis or C5 Activation. Arch Surg 123: 199-203 [Abstract]