Journal of Experimental Medicine, Vol 158, 857-871, Copyright © 1983 by Rockefeller University Press
Factor-dependent in vitro growth of human normal bone marrow-derived basophil-like cells
K Tadokoro, BM Stadler and AL De Weck
A factor(s) present in supernatants from lectin-stimulated peripheral blood
mononuclear cells promoted the production of basophil-like cells in liquid
cultures of normal human bone marrow cells. The cultured basophil-like
cells had lobulated or round nuclei, and the cytoplasmic granules stained
metachromatically with toluidine blue and azurophilic with Giemsa. 20% of
the metachromatically staining cells were peroxidase positive but not
positive for nonspecific esterase. The histamine content was 0.5-2 pg/cell.
The basophil-like cells released histamine upon challenge with calcium
ionophore A23187 but not with compound 48/80. They also released histamine
with anti-IgE when passively sensitized with human myeloma IgE. The
development of basophil-like cells was promoted in a dose-dependent fashion
by a factor(s) in the conditioned medium. Blocking of cell proliferation
with hydroxyurea or X irradiation inhibited the development of basophil-
like cells. The production of the factor was dependent on the presence of T
cells. The factor was different from interleukin 2 and its molecular weight
was estimated to be 25,000-40,000 by gel filtration on a Sephacryl S-200
column. Thus, human basophil-like cells derived from normal bone marrow
cells can grow and differentiate in vitro under the regulation of T cells.
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