Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells

doi:10.1084/jem.158.3.836

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Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells

J Kaye, S Porcelli, J Tite, B Jones and CA Janeway Jr

Two antisera and a monoclonal antibody raised in BALB.K mice against cloned, major histocompatibility complex (MHC)-restricted, antigen- specific helper T cell lines are described. These antibodies are specific for individual cloned T cell lines and are potent inducers of T cell proliferation. The induction of T cell proliferation by these antibodies requires the presence of an adherent accessory cell. There is no H-2 restriction between this accessory cell and the cloned T cell, nor is this antibody-induced proliferation blocked by a monoclonal anti-Fc receptor antibody. The requirement for an accessory cell, however, is eliminated in the presence of an IL-1- or IL-2-rich supernatant. Thus this system allows the analysis of helper T cell activation with only a single cell type present. Anti-T cell sera also induce T cell-dependent B cell proliferation and immunoglobulin secretion. The induction of T cell-dependent B cell activation by these sera does not require H-2-matched T cells and B cells. The specificity of these antibodies and their ability to stimulate cloned helper T cells in the absence of antigen and antigen-presenting cells strongly suggest that these antibodies are directed against antigen and/or Ia recognition sites on the T cell.
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Im, S.-H., Hueber, A., Monticelli, S., Kang, K.-H., Rao, A. (2004). Chromatin-level Regulation of the IL10 Gene in T Cells. J. Biol. Chem. 279: 46818-46825 [Abstract] [Full Text]
Boisvert, J., Edmondson, S., Krummel, M. F. (2004). Immunological Synapse Formation Licenses CD40-CD40L Accumulations at T-APC Contact Sites. J. Immunol. 173: 3647-3652 [Abstract] [Full Text]
Monticelli, S., Solymar, D. C., Rao, A. (2004). Role of NFAT Proteins in IL13 Gene Transcription in Mast Cells. J. Biol. Chem. 279: 36210-36218 [Abstract] [Full Text]
Baguet, A., Bix, M. (2004). Chromatin landscape dynamics of the Il4-Il13 locus during T helper 1 and 2 development. Proc. Natl. Acad. Sci. USA 101: 11410-11415 [Abstract] [Full Text]
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Schaefer, B. C., Kappler, J. W., Kupfer, A., Marrack, P. (2004). Complex and dynamic redistribution of NF-{kappa}B signaling intermediates in response to T cell receptor stimulation. Proc. Natl. Acad. Sci. USA 101: 1004-1009 [Abstract] [Full Text]
Tawill, S., Le Goff, L., Ali, F., Blaxter, M., Allen, J. E. (2004). Both Free-Living and Parasitic Nematodes Induce a Characteristic Th2 Response That Is Dependent on the Presence of Intact Glycans. Infect. Immun. 72: 398-407 [Abstract] [Full Text]
Niki, Y., Yamada, H., Kikuchi, T., Toyama, Y., Matsumoto, H., Fujikawa, K., Tada, N. (2004). Membrane-Associated IL-1 Contributes to Chronic Synovitis and Cartilage Destruction in Human IL-1{alpha} Transgenic Mice. J. Immunol. 172: 577-584 [Abstract] [Full Text]
Mo, R.-R., Eisenbraun, J. K., Sonstein, J., Craig, R. A., Curtis, J. L., Stoolman, L. M., Chen, J., Yung, R. L. (2003). CD49d Overexpression and T Cell Autoimmunity. J. Immunol. 171: 745-753 [Abstract] [Full Text]
Cantu, C. III, Benlagha, K., Savage, P. B., Bendelac, A., Teyton, L. (2003). The Paradox of Immune Molecular Recognition of {alpha}-Galactosylceramide: Low Affinity, Low Specificity for CD1d, High Affinity for {alpha}{beta} TCRs. J. Immunol. 170: 4673-4682 [Abstract] [Full Text]
Luhder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Muller, I., Winkler, E., Kerkau, T., Ikemizu, S., Davis, S. J., Hanke, T., Hunig, T. (2003). Topological Requirements and Signaling Properties of T Cell-activating, Anti-CD28 Antibody Superagonists. JEM 197: 955-966 [Abstract] [Full Text]
Blander, J. M., Sant'Angelo, D. B., Metz, D., Kim, S.-W., Flavell, R. A., Bottomly, K., Janeway, C. A. Jr. (2003). A Pool of Central Memory-Like CD4 T Cells Contains Effector Memory Precursors. J. Immunol. 170: 2940-2948 [Abstract] [Full Text]
Dao, T., Blander, J. M., Sant'Angelo, D. B. (2003). Recognition of a Specific Self-Peptide: Self-MHC Class II Complex Is Critical for Positive Selection of Thymocytes Expressing the D10 TCR. J. Immunol. 170: 48-54 [Abstract] [Full Text]
Moss, W. C., Irvine, D. J., Davis, M. M., Krummel, M. F. (2002). Quantifying signaling-induced reorientation of T cell receptors during immunological synapse formation. Proc. Natl. Acad. Sci. USA 99: 15024-15029 [Abstract] [Full Text]
Dorado, B., Jerez, M. J., Flores, N., Martin-Saavedra, F. M., Duran, C., Ballester, S. (2002). Autocrine IL-4 Gene Regulation at Late Phases of TCR Activation in Differentiated Th2 Cells. J. Immunol. 169: 3030-3037 [Abstract] [Full Text]
Sant'Angelo, D. B., Janeway, C. A. Jr. (2002). Negative selection of thymocytes expressing the D10 TCR. Proc. Natl. Acad. Sci. USA 99: 6931-6936 [Abstract] [Full Text]
Gallot, G., Vivien, R., Ibisch, C., Lule, J., Davrinche, C., Gaschet, J., Vie, H. (2001). Purification of Ag-Specific T Lymphocytes After Direct Peripheral Blood Mononuclear Cell Stimulation Followed by CD25 Selection. I. Application to CD4+ or CD8+ Cytomegalovirus Phosphoprotein pp65 Epitope Determination. J. Immunol. 167: 4196-4206 [Abstract] [Full Text]
Liu, J., Arai, K.-i., Arai, N. (2001). Inhibition of NFATx Activation by an Oligopeptide: Disrupting the Interaction of NFATx with Calcineurin. J. Immunol. 167: 2677-2687 [Abstract] [Full Text]
Cartmell, T, Luheshi, G N, Hopkins, S J, Rothwell, N J, Poole, S (2001). Role of endogenous interleukin-1 receptor antagonist in regulating fever induced by localised inflammation in the rat. J. Physiol. 531: 171-180 [Abstract] [Full Text]
Mei{beta}ner, U., Blum, H., Schnare, M., Rollinghoff, M., Gessner, A. (2001). A soluble form of the murine common {gamma} chain is present at high concentrations in vivo and suppresses cytokine signaling. Blood 97: 183-191 [Abstract] [Full Text]
Raman, V. S., Bal, V., Rath, S., George, A. (2000). Ligation of CD27 on Murine B Cells Responding to T-Dependent and T-Independent Stimuli Inhibits the Generation of Plasma Cells. J. Immunol. 165: 6809-6815 [Abstract] [Full Text]
Dittel, B. N., Janeway, C. A. Jr. (2000). Differential Sensitivity to Mutations in a Single Peptide by Two TCRs Having Identical {beta}-Chains and Closely Related {alpha}-Chains. J. Immunol. 165: 6334-6340 [Abstract] [Full Text]
Krummel, M. F., Sjaastad, M. D., Wülfing, C., Davis, M. M. (2000). Differential Clustering of CD4 and CD3zeta During T Cell Recognition. Science 289: 1349-1352 [Abstract] [Full Text]
Li, B., Yu, H., Zheng, W.-p., Voll, R., Na, S., Roberts, A. W., Williams, D. A., Davis, R. J., Ghosh, S., Flavell, R. A. (2000). Role of the Guanosine Triphosphatase Rac2 in T Helper 1 Cell Differentiation. Science 288: 2219-2222 [Abstract] [Full Text]
Blander, J. M., Sant'Angelo, D. B., Bottomly, K., Janeway, C. A. Jr. (2000). Alteration at a Single Amino Acid Residue in the T Cell Receptor {alpha} Chain Complementarity Determining Region 2 Changes the Differentiation of Naive Cd4 T Cells in Response to Antigen from T Helper Cell Type 1 (Th1) to Th2. JEM 191: 2065-2074 [Abstract] [Full Text]
Fernandez-Centeno, E., de Ojeda, G., Rojo, J. M., Portoles, P. (2000). Crry/p65, a Membrane Complement Regulatory Protein, Has Costimulatory Properties on Mouse T Cells. J. Immunol. 164: 4533-4542 [Abstract] [Full Text]
Nishikomori, R., Ehrhardt, R. O., Strober, W. (2000). T Helper Type 2 Cell Differentiation Occurs in the Presence of Interleukin 12 Receptor {beta}2 Chain Expression and Signaling. JEM 191: 847-858 [Abstract] [Full Text]
Reinherz, E. L., Tan, K., Tang, L., Kern, P., Liu, J., Xiong, Y., Hussey, R. E., Smolyar, A., Hare, B., Zhang, R., Joachimiak, A., Chang, H., Wagner, G., Wang, J. (1999). The Crystal Structure of a T Cell Receptor in Complex with Peptide and MHC Class II. Science 286: 1913-1921 [Abstract] [Full Text]
Chen, D., Heath, V., O'Garra, A., Johnston, J., McMahon, M. (1999). Sustained Activation of the Raf-MEK-ERK Pathway Elicits Cytokine Unresponsiveness in T Cells. J. Immunol. 163: 5796-5805 [Abstract] [Full Text]
Ashany, D., Savir, A., Bhardwaj, N., Elkon, K. B. (1999). Dendritic Cells Are Resistant to Apoptosis Through the Fas (CD95/APO-1) Pathway. J. Immunol. 163: 5303-5311 [Abstract] [Full Text]
Tandai-Hiruma, M., Endo, T., Kobata, A. (1999). Detection of Novel Carbohydrate Binding Activity of Interleukin-1. J. Biol. Chem. 274: 4459-4466 [Abstract] [Full Text]
Ho, I-C., Kim, J. H.-J., Rooney, J. W., Spiegelman, B. M., Glimcher, L. H. (1998). A potential role for the nuclear factor of activated T cells family of transcriptional regulatory proteins in adipogenesis. Proc. Natl. Acad. Sci. USA 95: 15537-15541 [Abstract] [Full Text]
Schnare, M., Blum, H., Juttner, S., Rollinghoff, M., Gessner, A. (1998). Specific Antagonism of Type I IL-4 Receptor with a Mutated Form of Murine IL-4. J. Immunol. 161: 3484-3492 [Abstract] [Full Text]
Singh, N., Bhatia, S., Abraham, R., Basu, S. K., George, A., Bal, V., Rath, S. (1998). Modulation of T Cell Cytokine Profiles and Peptide-MHC Complex Availability In Vivo by Delivery to Scavenger Receptors via Antigen Maleylation. J. Immunol. 160: 4869-4880 [Abstract] [Full Text]
Yasui, D. H., Genetta, T., Kadesch, T., Williams, T. M., Swain, S. L., Tsui, L. V., Huber, B. T. (1998). Transcriptional Repression of the IL-2 Gene in Th Cells by ZEB. J. Immunol. 160: 4433-4440 [Abstract] [Full Text]
Elliott, D. E., Blum, A. M., Li, J., Metwali, A., Weinstock, J. V. (1998). Preprosomatostatin Messenger RNA Is Expressed by Inflammatory Cells and Induced by Inflammatory Mediators and Cytokines. J. Immunol. 160: 3997-4003 [Abstract] [Full Text]
MacDonald, A. S., Maizels, R. M., Lawrence, R. A., Dransfield, I., Allen, J. E. (1998). Requirement for In Vivo Production of IL-4, But Not IL-10, in the Induction of Proliferative Suppression by Filarial Parasites. J. Immunol. 160: 4124-4132 [Abstract] [Full Text]
MacDonald, A. S., Maizels, R. M., Lawrence, R. A., Dransfield, I., Allen, J. E. (1998). Requirement for In Vivo Production of IL-4, But Not IL-10, in the Induction of Proliferative Suppression by Filarial Parasites. J. Immunol. 160: 1304-1312 [Abstract] [Full Text]
Khandekar, S. S., Bettencourt, B. M., Wyss, D. F., Naylor, J. W., Brauer, P. P., Huestis, K., Dwyer, D. S., Profy, A. T., Osburne, M. S., Banerji, J., Jones, B. (1997). Conformational Integrity and Ligand Binding Properties of a Single Chain T-cell Receptor Expressed in Escherichia coli. J. Biol. Chem. 272: 32190-32197 [Abstract] [Full Text]
Zhang, D.-H., Cohn, L., Ray, P., Bottomly, K., Ray, A. (1997). Transcription Factor GATA-3 Is Differentially Expressed in Murine Th1 and Th2 Cells and Controls Th2-specific Expression of the Interleukin-5 Gene. J. Biol. Chem. 272: 21597-21603 [Abstract] [Full Text]
Fields, B. A., Ober, B., Malchiodi, E. L., Lebedeva, M. I., Braden, B. C., Ysern, X., Kim, J.-K., Shao, X., Ward, E.S., Mariuzza, R. A. (1995). Crystal Structure of the V[IMAGE] Domain of a T Cell Antigen Receptor. Science 270: 1821-1824 [Abstract]
Pernis, A, Gupta, S, Gollob, K., Garfein, E, Coffman, R., Schindler, C, Rothman, P (1995). Lack of interferon gamma receptor beta chain and the prevention of interferon gamma signaling in TH1 cells. Science 269: 245-247 [Abstract]
Welham, M. J., Learmonth, L., Bone, H., Schrader, J. W. (1995). Interleukin-13 Signal Transduction in Lymphohemopoietic Cells. J. Biol. Chem. 270: 12286-12296 [Abstract] [Full Text]
Grandis, J. R., Chang, M.-J., Yu, W.-D., Johnson, C. S. (1995). Antitumor Activity of Interleukin-1{alpha} and Cisplatin in a Murine Model System. Arch Otolaryngol Head Neck Surg 121: 197-200 [Abstract]
Corr, M, Slanetz, A., Boyd, L., Jelonek, M., Khilko, S, al-Ramadi, B., Kim, Y., Maher, S., Bothwell, A., Margulies, D. (1994). T cell receptor-MHC class I peptide interactions: affinity, kinetics, and specificity. Science 265: 946-949 [Abstract]
Ertel, W., Morrison, M. H., Ayala, A., Chaudry, I. H. (1993). Modulation of Macrophage Membrane Phospholipids by n-3 Polyunsaturated Fatty Acids Increases Interleukin 1 Release and Prevents Suppression of Cellular Immunity Following Hemorrhagic Shock. Arch Surg 128: 15-21 [Abstract]
Kang, S., Tran, A., Grilli, M, Lenardo, M. (1992). NF-kappa B subunit regulation in nontransformed CD4+ T lymphocytes. Science 256: 1452-1456 [Abstract]
Moore, K., Vieira, P, Fiorentino, D., Trounstine, M., Khan, T., Mosmann, T. (1990). Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 248: 1230-1234 [Abstract]
Ford, H. R., Hoffman, R. A., Wing, E. J., Magee, D. M., McIntyre, L., Simmons, R. L. (1989). Characterization of Wound Cytokines in the Sponge Matrix Model. Arch Surg 124: 1422-1428 [Abstract]
Hurley, T., Luo, K, Sefton, B. (1989). Activators of protein kinase C induce dissociation of CD4, but not CD8, from p56lck. Science 245: 407-409 [Abstract]
Burkly, L.C., Lo, D., Kanagawa, O., Brinster, R.L., Flavell, R.A. (1989). Clonal Anergy of I-E-tolerant T Cells in Transgenic Mice with Pancreatic Expression of MHC Class II I-E. Cold Spring Harb Symp Quant Biol 54: 815-820 [Abstract]
Koning, F, Stingl, G, Yokoyama, W., Yamada, H, Maloy, W., Tschachler, E, Shevach, E., Coligan, J. (1987). Identification of a T3-associated gamma delta T cell receptor on Thy-1+ dendritic epidermal Cell lines. Science 236: 834-837 [Abstract]
Lew, A., Pardoll, D., Maloy, W., Fowlkes, B., Kruisbeek, A, Cheng, S., Germain, R., Bluestone, J., Schwartz, R., Coligan, J. (1986). Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. Science 234: 1401-1405 [Abstract]
Isobe, M, Erikson, J, Emanuel, B., Nowell, P., Croce, C. (1985). Location of gene for beta subunit of human T-cell receptor at band 7q35, a region prone to rearrangements in T cells. Science 228: 580-582 [Abstract]
Cannon, J., Dinarello, C. (1985). Increased plasma interleukin-1 activity in women after ovulation. Science 227: 1247-1249 [Abstract]
Croce, C., Isobe, M, Palumbo, A, Puck, J, Ming, J, Tweardy, D, Erikson, J, Davis, M, Rovera, G (1985). Gene for alpha-chain of human T-cell receptor: location on chromosome 14 region involved in T-cell neoplasms. Science 227: 1044-1047 [Abstract]
Fremont, D. H., Dai, S., Chiang, H., Crawford, F., Marrack, P., Kappler, J. (2002). Structural Basis of Cytochrome c Presentation by IEk. JEM 195: 1043-1052 [Abstract] [Full Text]
Glazier, K. S., Hake, S. B., Tobin, H. M., Chadburn, A., Schattner, E. J., Denzin, L. K. (2002). Germinal Center B Cells Regulate Their Capability to Present Antigen by Modulation of HLA-DO. JEM 195: 1063-1069 [Abstract] [Full Text]