Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation

doi:10.1084/jem.156.6.1860

This Article

Full Text (PDF, 460K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JEM

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Mayer, L.

Articles by Kunkel, H. G.

Search for Related Content

PubMed

PubMed Citation

Articles by Mayer, L.

Articles by Kunkel, H. G.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation

L Mayer, SM Fu and HG Kunkel

Human T-T hybridomas were established by fusion of concanavalin A- activated OKT-4+ T cells with hypoxanthine guanine phosphoribosyl transferase-deficient as well as nondeficient T cell lines. Four hybrids were selected for further study. Supernatant from hybrid clone J1.3 specifically enhanced IgA production and secretion by isolated human B cells, with increases in IgA plaque-forming cells approaching those seen with addition of autologous T cells and pokeweed mitogen. A monoclonal lymphocytic leukemia with membrane IgA also differentiated to IgA plasma cells by this supernatant. Evidence suggests that this hybrid supernatant acts on post-switch IgA-committed B cells. The other hybrids were not isotype specific; hybrid J2S1 enhanced polyclonal Ig secretion and hybrids K1 and K8 induced B cell proliferation without induction of Ig secretion.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Buendia, B, Santa-Maria, A, Courvalin, J. (1999). Caspase-dependent proteolysis of integral and peripheral proteins of nuclear membranes and nuclear pore complex proteins during apoptosis. J. Cell Sci. 112: 1743-1753 [Abstract]
Panja, A., Goldberg, S., Eckmann, L., Krishen, P., Mayer, L. (1998). The Regulation and Functional Consequence of Proinflammatory Cytokine Binding on Human Intestinal Epithelial Cells. J. Immunol. 161: 3675-3684 [Abstract] [Full Text]
Perret, E, Moudjou, M, Geraud, M., Derancourt, J, Soyer-Gobillard, M., Bornens, M (1995). Identification of an HSP70-related protein associated with the centrosome from dinoflagellates to human cells. J. Cell Sci. 108: 711-725 [Abstract]
Dominguez, J., Buendia, B, Lopez-Otin, C, Antony, C, Karsenti, E, Avila, J (1994). A protein related to brain microtubule-associated protein MAP1B is a component of the mammalian centrosome. J. Cell Sci. 107: 601-611 [Abstract]
CONLEY, M. E., DELACROIX, D. L. (1987). Intravascular and Mucosal Immunoglobulin A: Two Separate but Related Systems of Immune Defense?. ANN INTERN MED 106: 892-899 [Abstract]
Laurence, J, Mayer, L (1984). Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors. Science 225: 66-69 [Abstract]