Journal of Experimental Medicine, Vol 156, 1325-1334, Copyright © 1982 by Rockefeller University Press
I-J-restricted interactions in the generation of azobenzenearsonate- specific suppressor T cells
M Takaoki, MS Sy, A Tominaga, A Lowy, M Tsurufuji, R Finberg, B Benacerraf and MI Greene
The genetic restrictions of the activation of third-order suppressor cells
(Ts3) were studied in mice, using two different types of anti-
azobenzenearsonate (ABA)-immune responses, namely delayed-type
hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) generation. Ts2
cells were induced in several different strains of mice by injecting
monoclonal T hybridoma molecules or first-order suppressor factors (TsF1)
originating in A/J (H-2a, Igh-1e) mice and then testing the TsF2 molecules
derived from these Ts2 in A/J and A.By (H-2b, Igh-1e) or (A/J X A.By)F1
(H-2a/b, Igh-1e) and (C57Bl/6 X A/J)F1 (H-2b/a, Igh-1e) mice. It was shown
that the activity of TsF2 was restricted to the I-J of the strain in which
Ts2 was induced. By genetic analysis, restriction was shown to be due to
the requirement of H-2 identity between ABA-coupled cells used for Ts3
activation and the strain of the TsF2 origin. Moreover, by using
H-2-congenic ABA-coupled cells, we were also able to precisely map and
demonstrate that ABA-coupled cells I-J identical to TsF2 induced in various
strains were necessary for effective suppression to occur. This selective
activation of Ts3 suggested the existence of I-J-related antigen
presentation for suppression as the counterpart of I-A or
I-A-I-E-restricted antigen presentation for positive immune responses.
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