Journal of Experimental Medicine, Vol 155, 1579-1584, Copyright © 1982 by Rockefeller University Press

ARTICLES

Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies

U Landegren, U Ramstedt, I Axberg, M Ullberg, M Jondal and H Wigzell

Out of a panel of seven monoclonal antibodies with affinity for human lymphoid cells, three were shown to prevent cytotoxic T cell activity, whereas none affected natural killer cell activity when applied without complement. Anti-OKT3 and anti-Leu-2a, with affinity for all T cells and the cytotoxic/suppressive subset, respectively were both shown to inhibit T killing by their interaction with the effector cell. For anti- OKT3, the inhibition remained after free antibody was washed away. Anti- Leu-2a, in contrast, induced a rapidly reversible inhibition. Using a single cell assay, anti-OKT3 was shown to reduce the lytic ability without affecting target cell binding, whereas anti-Leu-2a prevented the effectors from binding target cells.
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• Spits, H, van Schooten, W, Keizer, H, van Seventer, G, van de Rijn, M, Terhorst, C, de Vries, J. (1986). Alloantigen recognition is preceded by nonspecific adhesion of cytotoxic T cells and target cells. Science 232: 403-405 [Abstract]  
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