Journal of Experimental Medicine, Vol 155, 1050-1062, Copyright © 1982 by Rockefeller University Press
Specificity studies on cytolytic T lymphocytes directed against murine leukemia virus-induced tumors. Analysis of monoclonal cytolytic T lymphocytes
F Plata
The specificities of cloned cytolytic T lymphocytes (CTL) were studied for
the analysis of CTL populations generated against murine leukemia viruses
(MuLV) in H-2 congenic BALB/c (H-2d) and BALB.B (H-2b) mice. In particular,
CTL generated in response to tumors induced by Gross MuLV and Friend MuLV
were studied; these tumors expressed virus-induced antigens that do not
cross-react and that can be distinguished from each other. The systematic
study of 92 CTL clones clearly indicated that MuLV-immune CTL were highly
heterogeneous with respect to both the intensities of target cell lysis
that they mediated and to their specificity of recognition of MuLV-induced
tumor target cells. Various categories of CTL clones were identified,
ranging from CTL clones tht were tightly H-2 restricted and specific for
the immunizing tumor to CTL clones that displayed no discernible patterns
of specificity and that attacked a large number of different target cells.
In addition, the surface markers of these cloned CTL were defined, and the
best conditions for their prolonged maintenance in culture were determined.
The present data indicate that future efforts in the definition of target
antigens recognized by tumor-specific CTL should be performed with
monoclonal lymphocytes.
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