Journal of Experimental Medicine, Vol 155, 937-942, Copyright © 1982 by Rockefeller University Press

ARTICLES

Intragenic recombination in an E beta gene for a murine Ia antigen

ML Plunkett, JE Coligan, CS David and JH Freed

The recombinant strain D2.GD was originally typed as I-Ad by serological methods. Indeed, the A alpha and A beta chains of the I-A antigens appear to exhibit normal behavior by the criteria of serology and two dimensional gel analysis. However, the E beta chain encoded by the I-A subregion of this strain, one of the two components of the plasma membrane located I-E antigens produced in D2.GD X A.TFR5)F1 animals, has been demonstrated to be the product of an intragenic recombinational event between E beta genes from the d and b haplotypes. Sequence analysis suggests that the amino-terminal portion of the Eg2 beta chain is derived from the d haplotype and, therefore, that the coding strand for this gene is oriented centromeric leads to telomeric (5' to 3' direction). Finally, these data combined with the data of Rose and Cullen (17) allow the ordering of the genes within the I-A subregion as (H-2K), A alpha, A beta, E beta ... (H-2D).
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This article has been cited by other articles:

• Kobori, J., Strauss, E, Minard, K, Hood, L (1986). Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science 234: 173-179 [Abstract]  
• Hood, L., Steinmetz, M., Goodenow, R., Eakle, K., Fisher, D., Kobori, J., Malissen, B., Malissen, M., McMillan, M., McNicholas, J., Orn, A., Pecht, M., Sher, B.T., Smith, L., Stroynowski, I., Sun, H., Winoto, A., Zuniga, M. (1983). Genes of the Major Histocompatibility Complex. Cold Spring Harb Symp Quant Biol 47: 1051-1065 [Abstract]  

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