Mitochondrial N-formylmethionyl proteins as chemoattractants for neutrophils

doi:10.1084/jem.155.1.264

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Mitochondrial N-formylmethionyl proteins as chemoattractants for neutrophils

H Carp

Mitochondria synthesize several hydrophobic proteins. Like bacteria, mitochondria initiate protein synthesis with an N-formylmethionine residue. Because N-formylmethionyl peptides have been found to be chemotactic for polymorphonuclear leukocytes (PMN), mitochondria isolated from cultured human cells and purified bovine mitochondrial proteins were tested for PMN chemotactic activity in vitro. Nondisrupted mitochondria were not chemotactic. However, intact mitochondria that had been incubated with a lysosomal lysate did stimulate PMN migration. Antibodies directed against two mitochondrial enzymes, cytochrome oxidase and ATPase, (both of which contain mitochondrially synthesized subunits) but not anti-C3 or anti-C5 decreased mitochondrially derived chemotactic activity. In addition, purified bovine mitochondrial N-formylmethionyl proteins stimulated PMN migration in vitro, whereas nonformylated mitochondrial proteins did not. Furthermore, the chemotactic activity of purified mitochondrial proteins and disrupted mitochondria was decreased by the formyl peptide antagonist butyloxycarbonyl-phenylalanine-leucine-phenylalanine-leucine- phenylalanine. Finally, disrupted mitochondria and purified mitochondrial proteins stimulated PMN-directed migration (chemotaxis), according to accepted criteria. In addition to other chemotactic factors, release of N-formylmethionyl proteins from mitochondria at sites of tissue damage, may play a role in the accumulation of inflammatory cells at these sites.
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Devosse, T., Guillabert, A., D'Haene, N., Berton, A., De Nadai, P., Noel, S., Brait, M., Franssen, J.-D., Sozzani, S., Salmon, I., Parmentier, M. (2009). Formyl Peptide Receptor-Like 2 Is Expressed and Functional in Plasmacytoid Dendritic Cells, Tissue-Specific Macrophage Subpopulations, and Eosinophils. J. Immunol. 182: 4974-4984 [Abstract] [Full Text]
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Southgate, E. L., He, R. L., Gao, J.-L., Murphy, P. M., Nanamori, M., Ye, R. D. (2008). Identification of Formyl Peptides from Listeria monocytogenes and Staphylococcus aureus as Potent Chemoattractants for Mouse Neutrophils. J. Immunol. 181: 1429-1437 [Abstract] [Full Text]
Babbin, B. A., Jesaitis, A. J., Ivanov, A. I., Kelly, D., Laukoetter, M., Nava, P., Parkos, C. A., Nusrat, A. (2007). Formyl Peptide Receptor-1 Activation Enhances Intestinal Epithelial Cell Restitution through Phosphatidylinositol 3-Kinase-Dependent Activation of Rac1 and Cdc42. J. Immunol. 179: 8112-8121 [Abstract] [Full Text]
Gao, J.-L., Guillabert, A., Hu, J., Le, Y., Urizar, E., Seligman, E., Fang, K. J., Yuan, X., Imbault, V., Communi, D., Wang, J. M., Parmentier, M., Murphy, P. M., Migeotte, I. (2007). F2L, a Peptide Derived from Heme-Binding Protein, Chemoattracts Mouse Neutrophils by Specifically Activating Fpr2, the Low-Affinity N-Formylpeptide Receptor. J. Immunol. 178: 1450-1456 [Abstract] [Full Text]
Chiang, N., Serhan, C. N., Dahlen, S.-E., Drazen, J. M., Hay, D. W. P., Rovati, G. E., Shimizu, T., Yokomizo, T., Brink, C. (2006). The Lipoxin Receptor ALX: Potent Ligand-Specific and Stereoselective Actions in Vivo. Pharmacol. Rev. 58: 463-487 [Abstract] [Full Text]
Edwards, B. S., Bologa, C., Young, S. M., Balakin, K. V., Prossnitz, E. R., Savchuck, N. P., Sklar, L. A., Oprea, T. I. (2005). Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists. Mol. Pharmacol. 68: 1301-1310 [Abstract] [Full Text]
Zhou, Y., Bian, X., Le, Y., Gong, W., Hu, J., Zhang, X., Wang, L., Iribarren, P., Salcedo, R., Howard, O. M. Z., Farrar, W., Wang, J. M. (2005). Formylpeptide Receptor FPR and the Rapid Growth of Malignant Human Gliomas. JNCI J Natl Cancer Inst 97: 823-835 [Abstract] [Full Text]
Haas, P.-J., de Haas, C. J. C., Kleibeuker, W., Poppelier, M. J. J. G., van Kessel, K. P. M., Kruijtzer, J. A. W., Liskamp, R. M. J., van Strijp, J. A. G. (2004). N-Terminal Residues of the Chemotaxis Inhibitory Protein of Staphylococcus aureus Are Essential for Blocking Formylated Peptide Receptor but Not C5a Receptor. J. Immunol. 173: 5704-5711 [Abstract] [Full Text]
Le, Y., Iribarren, P., Zhou, Y., Gong, W., Hu, J., Zhang, X., Wang, J. M. (2004). Silencing the Formylpeptide Receptor FPR by Short-Interfering RNA. Mol. Pharmacol. 66: 1022-1028 [Abstract] [Full Text]
Sun, R., Iribarren, P., Zhang, N., Zhou, Y., Gong, W., Cho, E. H., Lockett, S., Chertov, O., Bednar, F., Rogers, T. J., Oppenheim, J. J., Wang, J. M. (2004). Identification of Neutrophil Granule Protein Cathepsin G as a Novel Chemotactic Agonist for the G Protein-Coupled Formyl Peptide Receptor. J. Immunol. 173: 428-436 [Abstract] [Full Text]
Partida-Sanchez, S., Iribarren, P., Moreno-Garcia, M. E., Gao, J.-L., Murphy, P. M., Oppenheimer, N., Wang, J. M., Lund, F. E. (2004). Chemotaxis and Calcium Responses of Phagocytes to Formyl Peptide Receptor Ligands Is Differentially Regulated by Cyclic ADP Ribose. J. Immunol. 172: 1896-1906 [Abstract] [Full Text]
Svensson, L., Dahlgren, C., Wenneras, C. (2002). The chemoattractant Trp-Lys-Tyr-Met-Val-D-Met activates eosinophils through the formyl peptide receptor and one of its homologues, formyl peptide receptor-like 1. J. Leukoc. Biol. 72: 810-818 [Abstract] [Full Text]
Schroder, J.-M., Hasler, R., Grabowsky, J., Kahlke, B., Mallet, A. I. (2002). Identification of Diacylated Ureas as a Novel Family of Fungus-specific Leukocyte-activating Pathogen-associated Molecules. J. Biol. Chem. 277: 27887-27895 [Abstract] [Full Text]
Biondi, C., Pavan, B., Enrica Ferretti, M., Ginanni Corradini, F., Maria Neri, L., Vesce, F. (2001). Formyl-Methionyl-Leucyl-Phenylalanine Induces Prostaglandin E2 Release from Human Amnion-Derived WISH Cells by Phospholipase C-Mediated [Ca2+]i Rise. Biol. Reprod. 64: 865-870 [Abstract] [Full Text]
Wiley, J. A., Cerwenka, A., Harkema, J. R., Dutton, R. W., Harmsen, A. G. (2001). Production of Interferon-{{gamma}} by Influenza Hemagglutinin-Specific CD8 Effector T Cells Influences the Development of Pulmonary Immunopathology. Am. J. Pathol. 158: 119-130 [Abstract] [Full Text]
He, R., Tan, L., Browning, D. D., Wang, J. M., Ye, R. D. (2000). The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Is a Potent Chemotactic Agonist for Mouse Formyl Peptide Receptor. J. Immunol. 165: 4598-4605 [Abstract] [Full Text]
Shen, W., Li, B., Wetzel, M. A., Rogers, T. J., Henderson, E. E., Su, S. B., Gong, W., Le, Y., Sargeant, R., Dimitrov, D. S., Oppenheim, J. J., Wang, J. M. (2000). Down-regulation of the chemokine receptor CCR5 by activation of chemotactic formyl peptide receptor in human monocytes. Blood 96: 2887-2894 [Abstract] [Full Text]
Yang, D., Chen, Q., Stoll, S., Chen, X., Howard, O. M. Z., Oppenheim, J. J. (2000). Differential Regulation of Responsiveness to fMLP and C5a Upon Dendritic Cell Maturation: Correlation with Receptor Expression. J. Immunol. 165: 2694-2702 [Abstract] [Full Text]
Le, Y., Gong, W., Li, B., Dunlop, N. M., Shen, W., Su, S. B., Ye, R. D., Wang, J. M. (1999). Utilization of Two Seven-Transmembrane, G Protein-Coupled Receptors, Formyl Peptide Receptor-Like 1 and Formyl Peptide Receptor, by the Synthetic Hexapeptide WKYMVm for Human Phagocyte Activation. J. Immunol. 163: 6777-6784 [Abstract] [Full Text]
Hartt, J. K., Barish, G., Murphy, P. M., Gao, J.-L. (1999). N-Formylpeptides Induce Two Distinct Concentration Optima for Mouse Neutrophil Chemotaxis by Differential Interaction with Two N-Formylpeptide Receptor (Fpr) Subtypes: Molecular Characterization of Fpr2, a Second Mouse Neutrophil Fpr. JEM 190: 741-748 [Abstract] [Full Text]
Su, S. B., Gong, W.-h., Gao, J.-L., Shen, W.-P., Grimm, M. C., Deng, X., Murphy, P. M., Oppenheim, J. J., Wang, J. M. (1999). T20/DP178, an Ectodomain Peptide of Human Immunodeficiency Virus Type 1 gp41, Is an Activator of Human Phagocyte N-Formyl Peptide Receptor. Blood 93: 3885-3892 [Abstract] [Full Text]
Su, S. B., Gao, J.-l., Gong, W.-h., Dunlop, N. M., Murphy, P. M., Oppenheim, J. J., Wang, J. M. (1999). T21/DP107, A Synthetic Leucine Zipper-Like Domain of the HIV-1 Envelope gp41, Attracts and Activates Human Phagocytes by Using G-Protein-Coupled Formyl Peptide Receptors. J. Immunol. 162: 5924-5930 [Abstract] [Full Text]
Buzzi, M., Vesce, F., Ferretti, M. E., Fabbri, E., Biondi, C. (1999). Does Formyl-Methionyl-Leucyl-Phenylalanine Exert a Physiological Role in Labor in Women?. Biol. Reprod. 60: 1211-1216 [Abstract] [Full Text]
Edens, H. A., Parkos, C. A., Liang, T. W., Jesaitis, A. J., Cutler, J. E., Miettinen, H. M. (1999). Non-Serum-Dependent Chemotactic Factors Produced by Candida albicans Stimulate Chemotaxis by Binding to the Formyl Peptide Receptor on Neutrophils and to an Unknown Receptor on Macrophages. Infect. Immun. 67: 1063-1071 [Abstract] [Full Text]
Princiotta, M. F., Lenz, L. L., Bevan, M. J., Staerz, U. D. (1998). H2-M3 Restricted Presentation of a Listeria-derived Leader Peptide. JEM 187: 1711-1719 [Abstract] [Full Text]
Mills, J. S., Miettinen, H. M., Barnidge, D., Vlases, M. J., Wimer-Mackin, S., Dratz, E. A., Sunner, J., Jesaitis, A. J. (1998). Identification of a Ligand Binding Site in the Human Neutrophil Formyl Peptide Receptor Using a Site-specific Fluorescent Photoaffinity Label and Mass Spectrometry. J. Biol. Chem. 273: 10428-10435 [Abstract] [Full Text]
Sabeh, F., Hockberger, P., Sayeed, M. M. (1998). Signaling mechanisms of elevated neutrophil O⁻₂ generation after burn injury. Am. J. Physiol. Regul. Integr. Comp. Physiol. 274: R476-R485 [Abstract] [Full Text]
Tennenberg, S. D., Solomkin, J. S. (1988). Neutrophil Activation in Sepsis: The Relationship Between fMet-Leu-Phe Receptor Mobilization and Oxidative Activity. Arch Surg 123: 171-175 [Abstract]