Journal of Experimental Medicine, Vol 154, 1811-1826, Copyright © 1981 by Rockefeller University Press
Antigen receptors on murine T lymphocytes in contact sensitivity. I. Functional inhibition of effector T cells by monovalent 2,4- dinitrophenol: implication for a two-receptor model
JW Moorhead
Experiments were done to investigate the nature of the antigen receptor on
lymph node(LN) T cells from mice sensitized to 2,4- dinitrofluorobenzene
(DNFB). LN cells or purified T cells were treated in vitro with monovalent
or different multivalent 2,4-dinitrophenol (DNP) ligands. The effect of
this treatment was measured by testing the ability of the cells to transfer
contact sensitivity (CS) to DNFB into naive recipients. We found that
treatment of the T cells in vitro with either epsilon-DNP-L-lysine or
DNP-protein conjugates inhibits the transfer of CS in a dose-dependent way.
The inhibition is hapten specific and is not mediated by activation of
suppressor cells. Inhibition of the T cells by hapten in vitro is rapid
(15-30 min) and temperature independent but requires divalent cations in
the treatment medium. In addition, it was found that hapten-treated T cells
are unable to adsorb specific anti-idiotype antibody, and this inhibition
of adsorption is hapten specific. Collectively, these data indicate that
DNFB-immune T cells express a receptor specific for the hapten DNP and
provide evidence that supports a two-receptor model for T cell recognition
of antigen.
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