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Three cell lines of mature T cell origin derived from patients with cutaneous T cell lymphoma-leukemias (CTCL) were found to be constitutive producers of T cell growth factor (L-TCGF). These are the first reported human cell lines which constitutively produce TCGF. Biologically active TCGF could also be eluted from the surface of these cells using an acid glycine buffer under conditions that maintained cell viability, and subcellular fractionation showed that almost all the TCGF activity was associated with the plasma membrane. Over 30 other human hematopoietic cell lines derived from other disorders were unable to produce TCGF even after induction, and their acid eluates did not contain TCGF activity. L-TCGF from CTCL lines had the same biological activity as TCGF obtained from normal leukocytes (N-TCGF) in that they both supported the long-term growth of normal T cells only after the cells were previously activated by antigen or lectin. Both L-TCGF and N-TCGF increased the rate of proliferation of TCGF-independent and TCGF-dependent CTCL cell lines. The same three factor-independent cell lines that released TCGF adsorbed TCGF in a cell-concentration, time-, and temperature-dependent manner. Since the CTCL cell lines produce TCGF, adsorb TCGF, and increase their proliferative rate in response to TCGF or a related molecule, it is suggested that this endogenously produced factor plays a role in maintaining the abnormal proliferation of these cells in culture as permanently growing cell lines independent of exogenous TCGF. However, this does not mean that this is an essential aspect of neoplastic transformation. Since it is unusual to develop these cell lines in the absence of the continuous need for added TCGF, “autostimulation” may be one of the many unusual variant phenotypic properties sometimes associated with neoplastic cells that gives them a selective advantage for in vitro growth.
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• Newton-Nash, D. K., Newman, P. J. (1999). A New Role for Platelet-Endothelial Cell Adhesion Molecule-1 (CD31): Inhibition of TCR-Mediated Signal Transduction. J. Immunol. 163: 682-688 [Abstract] [Full Text]  
• Kieff, E. (1998). Current Perspectives on the Molecular Pathogenesis of Virus-Induced Cancers in Human Immunodeficiency Virus Infection and Acquired Immunodeficiency Syndrome. J Natl Cancer Inst Monogr 1998: 7-14 [Abstract] [Full Text]  
• Hassuneh, M. R., Nagarkatti, P. S., Nagarkatti, M. (1997). Evidence for the Participation of Interleukin-2 (IL-2) and IL-4 in the Regulation of Autonomous Growth and Tumorigenesis of Transformed Cells of Lymphoid Origin. Blood 89: 610-620 [Abstract] [Full Text]  
• Vachino, G., Chang, X.-J., Veldman, G. M., Kumar, R., Sako, D., Fouser, L. A., Berndt, M. C., Cumming, D. A. (1995). P-selectin Glycoprotein Ligand-1 Is the Major Counter-receptor for P-selectin on Stimulated T Cells and Is Widely Distributed in Non-functional Form on Many Lymphocytic Cells. J. Biol. Chem. 270: 21966-21974 [Abstract] [Full Text]  
• Ballard, D., Bohnlein, E, Lowenthal, J., Wano, Y, Franza, B., Greene, W. (1988). HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene. Science 241: 1652-1655 [Abstract]  
• Omary, M., Kagnoff, M. (1987). Identification of nuclear receptors for VIP on a human colonic adenocarcinoma cell line. Science 238: 1578-1581 [Abstract]  
• Greene, W., Leonard, W., Wano, Y, Svetlik, P., Peffer, N., Sodroski, J., Rosen, C., Goh, W., Haseltine, W. (1986). Trans-activator gene of HTLV-II induces IL-2 receptor and IL-2 cellular gene expression. Science 232: 877-880 [Abstract]  
• Greene, W.C., Depper, J.M., Kronke, M., Leonard, W.J. (1986). The Human Interleukin-2 Receptor: Role in Normal T-cell Growth and Association with HTLV-I-induced T-cell Leukemia. Cold Spring Harb Symp Quant Biol 51: 731-737 [Abstract]  
• Daniel, M., Letvin, N., King, N., Kannagi, M, Sehgal, P., Hunt, R., Kanki, P., Essex, M, Desrosiers, R. (1985). Isolation of T-cell tropic HTLV-III-like retrovirus from macaques. Science 228: 1201-1204 [Abstract]  
• BRODER, S., BUNN, P. A. Jr., JAFFE, E. S., BLATTNER, W., GALLO, R. C., WONG-STAAL, F., WALDMANN, T. A., DeVITA, V. T. Jr. (1984). T-Cell Lymphoproliferative Syndrome Associated with Human T-Cell Leukemia/Lymphoma Virus. ANN INTERN MED 100: 543-557 [Abstract]  
• Arya, S., Wong-Staal, F, Gallo, R. (1984). T-cell growth factor gene: lack of expression in human T-cell leukemia-lymphoma virus-infected cells. Science 223: 1086-1087 [Abstract]  
• Seigel, L., Harper, M., Wong-Staal, F, Gallo, R., Nash, W., O'Brien, S. (1984). Gene for T-cell growth factor: location on human chromosome 4q and feline chromosome B1. Science 223: 175-178 [Abstract]  
• Blattner, W. A., Takatsuki, K., Gallo, R. C. (1983). Human T-Cell Leukemia-Lymphoma Virus and Adult T-Cell Leukemia. JAMA 250: 1074-1080 [Abstract]  

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