Journal of Experimental Medicine, Vol 154, 1279-1289, Copyright © 1981 by Rockefeller University Press
Immune interferon produced to high levels by antigenic stimulation of human lymphocytes with influenza virus
FA Ennis and A Meager
Influenza virus stimulation of human lymphocytes induced high levels of
immune interferon in lymphocyte cultures. The lymphocytes of normal adults
produced approximately 1,000 U/10(6) cells, which was in large part gamma
interferon. The lymphocytes of individuals recently vaccinated yielded very
high levels (10-50,000 U/10(6) cells) of interferon. The interferon was pH
2 labile, and was not neutralized by antisera to alpha or beta interferon.
It did not bind to a monoclonal antibody to alpha interferon, and after
partial purification it had characteristics identical to human gamma
interferon induced by phytohemagglutinin. The highest yields were produced
by treatment of stimulator cells with live virus. Stimulation by whole
inactivated virus resulted in lower levels of interferon, and purified
hemagglutinin did not induce interferon. The antigen responsible for
stimulating the lymphocyte response and interferon induction is a cross-
reactive determinant present on all human and non-human influenza viruses
tested.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
