Journal of Experimental Medicine, Vol 153, 1236-1245, Copyright © 1981 by Rockefeller University Press
Priming of T helper cells by antigen-activated B cells. B cell-primed Lyt-1+ helper cells are restricted to cooperate with B cells expressing the IgvH phenotype of the priming B cells
J L'Age-Stehr
Activated B cells isolated shortly after primary immunization of BALB/c
donor mice with sheep erythrocytes (SRBC), were transferred to normal
syngeneic recipients or to low-dose cyclophosphamide-pretreated syngeneic
recipients. In pretreated recipients, the transfer of activated B cells,
but not of T cells or macrophages, resulted in an augmented production of
indirect plaque-forming cells in the primary immune response to SRBC but
not to horse erythrocytes. It was shown in double-transfer experiments that
T helper cells (Lyt-1+) had been stimulated by the transfer of
antigen-activated B cells. Criss-cross double-transfer experiments using
the mouse strains CB20 and BAB14 (congenic to BALB/c at the loci coding for
the immunoglobulin heavy chain) indicate that those T helper cells are
primed after recognition of B cell products that are encoded for by genes
linked to the loci coding for the variable region of the immunoglobulin
heavy chain (IgVH). The thus-primed Ig-dependent T helper cells (THIg) are
adaptively restricted to cooperate with B cells that display IgVH- linked
gene products similar to those that originally stimulated the THIg. These
findings suggest that in the course of an immune response to T
cell-dependent antigens, help for the production of specific IgG can be
provided by THIg that have been primed and/or clonally expanded after
recognition of IgVH-linked gene products by (e.g., complementary) T cell
receptors.
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