ARTICLES

Recent evidence, has demonstrated an association between a subpopulation of peripheral blood mononuclear cells, morphologically identified as large granular lymphocytes (LGL), and natural killer (NK) activity. We have now evaluated more directly the role of LGL in both NK activity and antibody- dependent cellular cytotoxicity (ADCC), by using highly enriched populations of LGL, obtained by centrifugation of peripheral blood mononuclear cells on Percoll discontinuous density gradients. Both spontaneous and interferon- augmented NK and ADCC activities were exclusively associated with the LGL- enriched, low density fractions. The majority of LGL formed conjugates with NK-susceptible and antibody-coated target cells. Approximately 20 percent of small conventional lymphocytes also formed conjugates with the target cells for NK, but this was not associated with cytotoxic activity. Virtually all LGL were found to have receptors for the Fc portion of IgG (FcR). The frequency of LGL among blood leukocytes was 2-6 percent. LGL could be enriched to an average purity of 95 percent by combining discontinuous density gradient centrifugation with subsequent adsorptions of the low density fractions on monolayers of immobilized immune complexes. About 50 percent of LGL were found to be FcR-bearing T cells (T(G)), forming low affinity rosettes with sheep erythrocytes at 4 degrees C. Only 10-20 percent of LGL formed high affinity rosettes with sheep erythrocytes at 29 degrees C. LGL could be enriched to a purity of more than 90 percent by depleting high affinity rosette-forming cells from low density Percoll fractions. LGL were only a subpopulation of T(G) cells, because some lymphocytes with conventional morphology also adhered to the immobilized immune complex monolayers and formed high affinity rosettes with sheep erythrocytes. Separation of these cells from LGL by discontinuous density gradient centrifugation indicated that they are not cytotoxic, suggesting a morphological and functional subdivision of T(G) cells. The verification in this study that virtually all human NK and K cells have a characteristic morphology adds a useful parameter to the monitoring of human lymphocytes, and the ability to purify these cells by simple physical procedures should be invaluable in their further characterization.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Huang, Y.-C., Hung, S.-W., Jan, T.-R., Liao, K.-W., Cheng, C.-H., Wang, Y.-S., Chu, R.-M. (2008). CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells. J. Leukoc. Biol. 84: 1501-1510 [Abstract] [Full Text]  
• Mailliard, R. B., Alber, S. M., Shen, H., Watkins, S. C., Kirkwood, J. M., Herberman, R. B., Kalinski, P. (2005). IL-18-induced CD83+CCR7+ NK helper cells. JEM 202: 941-953 [Abstract] [Full Text]  
• Fernandez, R. G., Leehan, J. A., Pastrana, R. F., Muniz, R. O. (2005). Effect of Malnutrition on K+ Current in T Lymphocytes. CVI 12: 808-813 [Abstract] [Full Text]  
• Hodge, D. L., Schill, W. B., Wang, J. M., Blanca, I., Reynolds, D. A., Ortaldo, J. R., Young, H. A. (2002). IL-2 and IL-12 Alter NK Cell Responsiveness to IFN-{gamma}-Inducible Protein 10 by Down-Regulating CXCR3 Expression. J. Immunol. 168: 6090-6098 [Abstract] [Full Text]  
• Blanca, I. R., Bere, E. W., Young, H. A., Ortaldo, J. R. (2001). Human B Cell Activation by Autologous NK Cells Is Regulated by CD40-CD40 Ligand Interaction: Role of Memory B Cells and CD5+ B Cells. J. Immunol. 167: 6132-6139 [Abstract] [Full Text]  
• Zhang, X., Wang, J. M., Gong, W. H., Mukaida, N., Young, H. A. (2001). Differential Regulation of Chemokine Gene Expression by 15-Deoxy-{{Delta}}12,1412,14 Prostaglandin J2. J. Immunol. 166: 7104-7111 [Abstract] [Full Text]  
• Liu, J. H., Wei, S., Lamy, T., Epling-Burnette, P. K., Starkebaum, G., Djeu, J. Y., Loughran, T. P. Jr (2000). Chronic neutropenia mediated by Fas ligand. Blood 95: 3219-3222 [Abstract] [Full Text]  
• Yu, C.-R., Ortaldo, J. R., Curiel, R. E., Young, H. A., Anderson, S. K., Gosselin, P. (1999). Role of a STAT Binding Site in the Regulation of the Human Perforin Promoter. J. Immunol. 162: 2785-2790 [Abstract] [Full Text]  
• YONEDA, T., ELLNER, J. J. (1998). CD4+ T Cell and Natural Killer Cell-dependent Killing of Mycobacterium tuberculosis by Human Monocytes. Am. J. Respir. Crit. Care Med. 158: 395-403 [Abstract] [Full Text]  
• Yu, C.-R., Kirken, R. A., Malabarba, M. G., Young, H. A., Ortaldo, J. R. (1998). Differential Regulation of the Janus Kinase-STAT Pathway and Biologic Function of IL-13 in Primary Human NK and T Cells: A Comparative Study with IL-4. J. Immunol. 161: 218-227 [Abstract] [Full Text]  
• Krenacs, L., Wellmann, A., Sorbara, L., Himmelmann, A. W., Bagdi, E., Jaffe, E. S., Raffeld, M. (1997). Cytotoxic Cell Antigen Expression in Anaplastic Large Cell Lymphomas of T- and Null-Cell Type and Hodgkin's Disease: Evidence for Distinct Cellular Origin. Blood 89: 980-989 [Abstract] [Full Text]  
• Somersalo, K., Carpén, O., Saksela, E., Gahmberg, C. G., Nortamo, P., Timonen, T. (1995). Activation of Natural Killer Cell Migration by Leukocyte Integrin-binding Peptide from Intracellular Adhesion Molecule-2 (ICAM-2). J. Biol. Chem. 270: 8629-8636 [Abstract] [Full Text]  
• Nissila, M., Salo, M., Granberg, C., Perttila, J., Neuvonen, P. (1988). Activity of Natural Killer Cells after Postoperative Amino Acid Infusion. JPEN J Parenter Enteral Nutr 12: 346-350 [Abstract]  
• Tartter, P. I., Steinberg, B., Barron, D. M., Martinelli, G. (1987). The Prognostic Significance of Natural Killer Cytotoxicity in Patients With Colorectal Cancer. Arch Surg 122: 1264-1268 [Abstract]  
• Siegel, J., Sharon, M, Smith, P., Leonard, W. (1987). The IL-2 receptor beta chain (p70): role in mediating signals for LAK, NK, and proliferative activities. Science 238: 75-78 [Abstract]  
• Strome, M., Clark, J. R., Fried, M. P., Rodliff, S., Blazar, B. A. (1987). T-Cell Subsets and Natural Killer Cell Function With Squamous Cell Carcinoma of the Head and Neck. Arch Otolaryngol Head Neck Surg 113: 1090-1093 [Abstract]  
• Orman, S. V., Schechter, G. P., Whang-Peng, J., Guccion, J., Chan, C., Schulof, R. S., Shalhoub, R. J. (1986). Nephrotic Syndrome Associated With a Clonal T-Cell Leukemia of Large Granular Lymphocytes With Cytotoxic Function. Arch Intern Med 146: 1827-1829 [Abstract]  
• Chan, W. C., Winton, E. F., Waldmann, T. A. (1986). Lymphocytosis of Large Granular Lymphocytes. Arch Intern Med 146: 1201-1203 [Abstract]  
• Buckley, R. H. (1986). Advances in the Diagnosis and Treatment of Primary Immunodeficiency Diseases. Arch Intern Med 146: 377-384 [Abstract]  
• WALLIS, W. J., LOUGHRAN, T. P. Jr., KADIN, M. E., CLARK, E. A., STARKEBAUM, G. A. (1985). Polyarthritis and Neutropenia Associated with Circulating Large Granular Lymphocytes. ANN INTERN MED 103: 357-362 [Abstract]  
• Ritz, J, Campen, T., Schmidt, R., Royer, H., Hercend, T, Hussey, R., Reinherz, E. (1985). Analysis of T-cell receptor gene rearrangement and expression in human natural killer clones. Science 228: 1540-1543 [Abstract]  
• LOUGHRAN, T. P. Jr., KADIN, M. E., STARKEBAUM, G., ABKOWITZ, J. L., CLARK, E. A., DISTECHE, C., LUM, L. G., SLICHTER, S. J. (1985). Leukemia of Large Granular Lymphocytes: Association with Clonal Chromosomal Abnormalities and Autoimmune Neutropenia, Thrombocytopenia, and Hemolytic Anemia. ANN INTERN MED 102: 169-175 [Abstract]  
• Herberman, R., Ortaldo, JR (1981). Natural killer cells: their roles in defenses against disease. Science 214: 24-30 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS