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Journal of Experimental Medicine, Vol 153, 488-493, Copyright © 1981 by Rockefeller University Press
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HS Lin, S Gordon, DM Chen and M Kurtz
We investigated the time-course involved in the conversion of mouse blood monocytes in vitro in cells capable of anchorage-independent growth. Two criteria were used to define when monocytes were fully converted to cells similar to mononuclear phagocytes present in inflammatory exudate, such as thioglycollate medium (TM)-elicited peritoneal exudate. They were the production of high levels of plasminogen activators and an ability to undergo anchorage-independent growth. Resident peritoneal macrophages were used as controls and for comparison. Our studies indicated that monocytes, but not resident peritoneal macrophages, could be converted to cells similar to TM- elicited mononuclear phagocytes after 2 d in culture.
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