A monoclonal antibody that recognizes B cells and B cell precursors in mice

doi:10.1084/jem.153.2.269

This Article

	Full Text (PDF, 784K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Coffman, R. L.
	Articles by Weissman, I. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Coffman, R. L.
	Articles by Weissman, I. L.


Social Bookmarking

	What's this?

ARTICLES

A monoclonal antibody that recognizes B cells and B cell precursors in mice

RL Coffman and IL Weissman

The monoclonal antibody, RA3-2C2, appears to be specific for cells within the B cell lineage. This antibody does not recognize thymocytes, peripheral T cells, or nonlymphoid hematopoietic cells in the spleen or bone marrow. Nor does it recognize the pluripotent hematopoietic stem cells, the spleen colony-forming unit, All sIg+ B cells and most plasma cells are RA3-2C2+. In addition, approximately 20% of nucleated bone marrow cells are RA3-2C2+ but sIg-. This population contains B cell precursors that can give rise to sIg+ cells within 2 d in vitro.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Oliveira, F. L, Chammas, R., Ricon, L., Fermino, M. L, Bernardes, E. S, Hsu, D. K, Liu, F.-T., Borojevic, R., El-Cheikh, M. C (2009). Galectin-3 regulates peritoneal B1-cell differentiation into plasma cells. Glycobiology 19: 1248-1258 [Abstract] [Full Text]
Weissman, I. L., Shizuru, J. A. (2008). The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases. Blood 112: 3543-3553 [Abstract] [Full Text]
Vondenhoff, M. F. R., Desanti, G. E., Cupedo, T., Bertrand, J. Y., Cumano, A., Kraal, G., Mebius, R. E., Golub, R. (2008). Separation of splenic red and white pulp occurs before birth in a LT{alpha}{beta}-independent manner. J. Leukoc. Biol. 84: 152-161 [Abstract] [Full Text]
Xu, Y., Beavitt, S.-J. E., Harder, K. W., Hibbs, M. L., Tarlinton, D. M. (2002). The Activation and Subsequent Regulatory Roles of Lyn and CD19 After B Cell Receptor Ligation are Independent. J. Immunol. 169: 6910-6918 [Abstract] [Full Text]
Martinez-M., J. A., Minguet, S., Gonzalo, P., Soro, P. G., de Andres, B., Izcue, A., Marcos, M. A. R., Gaspar, M.-L. (2001). Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice. Blood 98: 1862-1871 [Abstract] [Full Text]
Muerkoster, S., Laman, J. D., Rocha, M., Umansky, V., Schirrmacher, V. (2000). Functional and in Situ Evidence for Nitric Oxide Production Driven by CD40-CD40L Interactions in Graft-versus-Leukemia Reactivity. Clin. Cancer Res. 6: 1988-1996 [Abstract] [Full Text]
van Ewijk, W, Hollander, G, Terhorst, C, Wang, B (2000). Stepwise development of thymic microenvironments in vivo is regulated by thymocyte subsets. Development 127: 1583-1591 [Abstract]
Payne, K. J., Medina, K. L., Kincade, P. W. (1999). Loss of c-kit Accompanies B-Lineage Commitment and Acquisition of CD45R by Most Murine B-Lymphocyte Precursors. Blood 94: 713-723 [Abstract] [Full Text]
Le Bon, A., Wache, A.-C., Papiernik, M. (1999). In vivo elimination of viral superantigen-activated CD4+ T cells: apoptosis occurs at a distance from the activation site. Int Immunol 11: 373-382 [Abstract] [Full Text]
AKASHI, K., KONDO, M., CHESHIER, S., SHIZURU, J., GANDY, K., DOMEN, J., MEBIUS, R., TRAVER, D., WEISSMAN, I.L. (1999). Lymphoid Development from Stem Cells and the Common Lymphocyte Progenitors. Cold Spring Harb Symp Quant Biol 64: 1-12 [Abstract]
Gilfillan, S., Shen, X., Konig3, R. (1998). Selection and Function of CD4+ T Lymphocytes in Transgenic Mice Expressing Mutant MHC Class II Molecules Deficient in Their Interaction with CD4. J. Immunol. 161: 6629-6637 [Abstract] [Full Text]
Bonorino, C., Nardi, N. B., Zhang, X., Wysocki, L. J. (1998). Characteristics of the Strong Antibody Response to Mycobacterial Hsp70: A Primary, T Cell-Dependent IgG Response with no Evidence of Natural Priming or {gamma}{delta} T Cell Involvement. J. Immunol. 161: 5210-5216 [Abstract] [Full Text]
Sharma, M. D., Leite de Moraes, M., Zavala, F., Pontoux, C., Papiernik, M. (1998). Induction and Inhibition of CD40-CD40 Ligand Interactions: A New Strategy Underlying Host-Virus Relationships. J. Immunol. 161: 5357-5365 [Abstract] [Full Text]
Peakman, M.-C., Maizels, N. (1998). Localization of Splenic B Cells Activated for Switch Recombination by In Situ Hybridization with I{gamma}1 Switch Transcript and Rad51 Probes. J. Immunol. 161: 4008-4015 [Abstract] [Full Text]
Keller, G., Wall, C., Fong, A. Z.C., Hawley, T. S., Hawley, R. G. (1998). Overexpression of HOX11 Leads to the Immortalization of Embryonic Precursors With Both Primitive and Definitive Hematopoietic Potential. Blood 92: 877-887 [Abstract] [Full Text]
Bowman, T, Symonds, H, Gu, L, Yin, C, Oren, M, Van Dyke, T (1996). Tissue-specific inactivation of p53 tumor suppression in the mouse.. Genes Dev. 10: 826-835 [Abstract]
Corcoran, L M, Karvelas, M, Nossal, G J, Ye, Z S, Jacks, T, Baltimore, D (1993). Oct-2, although not required for early B-cell development, is critical for later B-cell maturation and for postnatal survival.. Genes Dev. 7: 570-582 [Abstract]
Witmer-Pack, M., Crowley, M., Inaba, K, Steinman, R. (1993). Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J. Cell Sci. 105: 965-973 [Abstract]