Journal of Experimental Medicine, Vol 152, 1571-1582, Copyright © 1980 by Rockefeller University Press
Antigen-specific helper T cells required for dominant production of an idiotype (THId) are not under immune response (Ir) gene control
K Bottomly and PH Maurer
Responder and nonresponder mice primed with poly-(L-glutamic acid,L-
lysine,L-phenylalanine) (GLPhe), the response to which is under the control
of immune response (Ir) genes, were used as a source of both types of
helper T cells required for a T15 idiotype dominated T- dependent
anti-phosphorylcholine (PC) response. It was found that the activity of one
of the helper T cells needed for an anti-PC response was under major
histocompatibility complex (MHC)-linked Ir gene control, and only
GLPhe-primed responder mice could be used as a source of these cells. These
T cells (ThMHC) whose presence is required for in vivo T-B collaboration
are found in normal and anti-mu-treated mice, and their activity depends on
the hapten being physically linked to the carrier molecule. By contrast,
the activity of the second helper T cell (ThId) required for a
T15-dominated anti-PC response was present in both GLPhe-primed responder
and nonresponder mice. The ThId cell set that is missing or deficient in
anti-mu treated mice can be restored by the addition of T cells from
normal, carrier-primed donors and restimulating with the priming carrier.
When T cells from GLPhe-primed donors are used as a source of ThId cells,
both responder and nonresponder donors provide helper cells capable of
inducing syngeneic B cells to produce a T15 dominated anti-Pc response.
These results are interpreted to suggest that idiotype recognizing helper T
cells (ThId) recognize antigen independent of known Ir gene products.
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