Mast cell heparin stimulates migration of capillary endothelial cells in vitro

doi:10.1084/jem.152.4.931

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Mast cell heparin stimulates migration of capillary endothelial cells in vitro

RG Azizkhan, JC Azizkhan, BR Zetter and J Folkman

Migration of capillary endothelial cells is an important component of angiogenesis in vivo. Increased numbers of mast cells have been associated with several types of angiogenesis. We have used a quantitative assay in vitro to demonstrate that mast cells release a factor that significantly increases bovine capillary endothelial cell migration. The factor is present in medium conditioned by mast cells as well as lysates of mast cells. The stimulatory effect of mast cells on migration is specific for capillary endothelial cells. Furthermore, mast cells have no mitogenic activity for capillary endothelial cells. Of all the secretory products of mast cells tested, only heparin stimulated capillary endothelial cell migration in vitro. Heparin preparations from a variety of sources stimulated capillary endothelial cell migration to the same degree but did not stimulate migration of several other cell types. The migration activity of heparin and mast cell conditioned medium was blocked by specific antagonists of heparin (protamine and heparinase), but not by chondroitinase ABC. The migration activity of mast cell conditioned medium was resistant to heat (100 degrees C) and incubation with proteolytic enzymes. These results suggest that the role of mast cells in angiogenesis may be to enhance migration of the endothelial cells of growing capillaries.
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Mitsi, M., Forsten-Williams, K., Gopalakrishnan, M., Nugent, M. A. (2008). A Catalytic Role of Heparin within the Extracellular Matrix. J. Biol. Chem. 283: 34796-34807 [Abstract] [Full Text]
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Nakayama, T., Mutsuga, N., Yao, L., Tosato, G. (2006). Prostaglandin E2 promotes degranulation-independent release of MCP-1 from mast cells. J. Leukoc. Biol. 79: 95-104 [Abstract] [Full Text]
Chetta, A., Zanini, A., Foresi, A., Del Donno, M., Castagnaro, A., D'Ippolito, R., Baraldo, S., Testi, R., Saetta, M., Olivieri, D. (2003). Vascular Component of Airway Remodeling in Asthma Is Reduced by High Dose of Fluticasone. Am. J. Respir. Crit. Care Med. 167: 751-757 [Abstract] [Full Text]
Tateno, S., Terai, M., Niwa, K., Jibiki, T., Hamada, H., Yasukawa, K., Honda, T., Oana, S., Kohno, Y. (2001). Alleviation of Myocardial Ischemia After Kawasaki Disease by Heparin and Exercise Therapy. Circulation 103: 2591-2597 [Abstract] [Full Text]
Bree, A. F., Siegfried, E., Sotelo-Avila, C., Nahass, G. (2001). Infantile Hemangiomas: Speculation on Placental Trophoblastic Origin. Arch Dermatol 137: 573-577 [Abstract] [Full Text]
Tomita, M., Matsuzaki, Y., Onitsuka, T. (2000). Effect of mast cells on tumor angiogenesis in lung cancer. Ann. Thorac. Surg. 69: 1686-1690 [Abstract] [Full Text]
Fujita, M. (2000). Heparin and angiogenic therapy. Eur Heart J 21: 270-274
Ignatescu, M. C., Gharehbaghi-Schnell, E., Hassan, A., Rezaie-Majd, S., Korschineck, I., Schleef, R. R., Glogar, H. D., Lang, I. M. (1999). Expression of the Angiogenic Protein, Platelet-Derived Endothelial Cell Growth Factor, in Coronary Atherosclerotic Plaques : In Vivo Correlation of Lesional Microvessel Density and Constrictive Vascular Remodeling. Arterioscler. Thromb. Vasc. Bio. 19: 2340-2347 [Abstract] [Full Text]
Zhang, P. J., Kobos, J. W., Brooks, J. J. (1999). The Prognostic Significance of Mast Cell Count in Soft Tissue Sarcomas. INT J SURG PATHOL 7: 141-148 [Abstract]
Lagunoff, D., Rickard, A. (1999). Mast Cell Granule Heparin Proteoglycan Induces Lacunae in Confluent Endothelial Cell Monolayers. Am. J. Pathol. 154: 1591-1600 [Abstract] [Full Text]
Shimizu, H., Hosokawa, H., Ninomiya, H., Miner, J. H., Masaki, T. (1999). Adhesion of Cultured Bovine Aortic Endothelial Cells to Laminin-1 Mediated by Dystroglycan. J. Biol. Chem. 274: 11995-12000 [Abstract] [Full Text]
Gengrinovitch, S., Berman, B., David, G., Witte, L., Neufeld, G., Ron, D. (1999). Glypican-1 Is a VEGF165 Binding Proteoglycan That Acts as an Extracellular Chaperone for VEGF165. J. Biol. Chem. 274: 10816-10822 [Abstract] [Full Text]
Boesiger, J., Tsai, M., Maurer, M., Yamaguchi, M., Brown, L. F., Claffey, K. P., Dvorak, H. F., Galli, S. J. (1998). Mast Cells Can Secrete Vascular Permeability Factor/ Vascular Endothelial Cell Growth Factor and Exhibit Enhanced Release after Immunoglobulin E-dependent Upregulation of Fc{varepsilon} Receptor I Expression. JEM 188: 1135-1145 [Abstract] [Full Text]
Grützkau, A., Krüger-Krasagakes, S., Baumeister, H., Schwarz, C., Kögel, H., Welker, P., Lippert, U., Henz, B. M., Möller, A. (1998). Synthesis, Storage, and Release of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF) by Human Mast Cells: Implications for the Biological Significance of VEGF206. Mol. Biol. Cell 9: 875-884 [Abstract] [Full Text]
Bombardini, T., Picano, E., Bombardini, T. (1997). The Coronary Angiogenetic Effect of Heparin: Experimental Basis and Clinical Evidence. ANGIOLOGY 48: 969-976 [Abstract]
Cook, J. L., Dzubow, L. M. (1997). Aging of the Skin: Implications for Cutaneous Surgery. Arch Dermatol 133: 1273-1277 [Abstract]
Silvagno, F., Follenzi, A., Arese, M., Prat, M., Giraudo, E., Gaudino, G., Camussi, G., Comoglio, P. M., Bussolino, F. (1995). In Vivo Activation of met Tyrosine Kinase by Heterodimeric Hepatocyte Growth Factor Molecule Promotes Angiogenesis. Arterioscler. Thromb. Vasc. Bio. 15: 1857-1865 [Abstract] [Full Text]
Broadway, D. C., Grierson, I., O'Brien, C., Hitchings, R. A. (1994). Adverse Effects of Topical Antiglaucoma Medication: I. The Conjunctival Cell Profile. Arch Ophthalmol 112: 1437-1445 [Abstract]
Bertolami, C. N., Messadi, D. V. (1994). The Role of Proteoglycans in Hard and Soft Tissue Repair. CROBM 5: 311-337 [Abstract] [Full Text]
Claman, H. N. (1989). On Scleroderma: Mast Cells, Endothelial Cells, and Fibroblasts. JAMA 262: 1206-1209 [Abstract]
Folkman, J, Weisz, P., Joullie, M., Li, W., Ewing, W. (1989). Control of angiogenesis with synthetic heparin substitutes. Science 243: 1490-1493 [Abstract]
Terranova, V.P., Wikesjo, U.M.E. (1988). Chemotaxis of Cells Isolated from Periodontal Tissues to Different Biological Response Modifiers. ADR 2: 215-222 [Abstract]
Glaser, B. M. (1988). Extracellular Modulating Factors and the Control of Intraocular Neovascularization: An Overview. Arch Ophthalmol 106: 603-607 [Abstract]
Atkins, F. M., Clark, R. A. F. (1987). Mast Cells and Fibrosis. Arch Dermatol 123: 191-193 [Abstract]
Folkman, J, Klagsbrun, M (1987). Angiogenic factors. Science 235: 442-447 [Abstract]
Majewski, S., Kaminski, M., Jablonska, S., Szmurlo, A., Pawinska, M. (1985). Angiogenic Capability of Peripheral Blood Mononuclear Cells in Psoriasis. Arch Dermatol 121: 1018-1021 [Abstract]
Maciag, T, Mehlman, T, Friesel, R, Schreiber, A. (1984). Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain. Science 225: 932-935 [Abstract]
Tilson, M. D., Fieg, E. L., Harvey, M. (1984). Malignant Neoplasia in Patients With Abdominal Aortic Aneurysms. Arch Surg 119: 792-794 [Abstract]
Thornton, S., Mueller, S., Levine, E. (1983). Human endothelial cells: use of heparin in cloning and long-term serial cultivation. Science 222: 623-625 [Abstract]
Folkman, J, Langer, R, Linhardt, R., Haudenschild, C, Taylor, S (1983). Angiogenesis inhibition and tumor regression caused by heparin or a heparin fragment in the presence of cortisone. Science 221: 719-725 [Abstract]