Synergistic genetic defect in B-lymphocyte function. I. Defective responses to B-cell stimulants and their genetic basis

doi:10.1084/jem.151.1.224

This Article

	Full Text (PDF, 624K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Bona, C.
	Articles by Paul, W. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Bona, C.
	Articles by Paul, W. E.


Social Bookmarking

	What's this?

ARTICLES

Synergistic genetic defect in B-lymphocyte function. I. Defective responses to B-cell stimulants and their genetic basis

C Bona, JJ Mond and WE Paul

CBA/N female mice, which express an X-linked defect in B-lymphocyte function, were mated with C3H/HeJ male mice, which are unresponsive to lipopolysaccharide (LPS). The resulting F1 hybrid females were mated to C3H/HeJ males. Approximately one-half of the backcross (BC.1) males obtained from this mating expressed a more profound immunologic defect than either of the parental strains. Spleen cells from these mice were unresponsive to a series of B-cell mitogens including LPS prepared from Escherichia coli K235 and from E. coli 0111:B4, lipoprotein mitogen from E. coli, and Nocardia water-soluble mitogen (NWSM). They failed to give in vitro antibody responses to the thymus-independent type 2 (TI- 2) antigen trinophenylated Ficoll and most were unresponsive to the TI- 1 antigens trinitrophenylated Brucella abortus, trinitrophenylated LPS, and trinitrophenylated NWSM. This synergistic defect in B-lymphocyte function depended on the presence of the CBA/N xid gene but the critical gene(s) from the C3H strain was not the defective Lps gene (Lpsd). These mice should provide a valuable tool for the elucidation of B-lymphocyte ontogeny, heterogeneity, and function.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Cabatingan, M. S., Schmidt, M. R., Sen, R., Woodland, R. T. (2002). Naive B Lymphocytes Undergo Homeostatic Proliferation in Response to B Cell Deficit. J. Immunol. 169: 6795-6805 [Abstract] [Full Text]
Bajpai, U. D., Zhang, K., Teutsch, M., Sen, R., Wortis, H. H. (2000). Bruton's Tyrosine Kinase Links the B Cell Receptor to Nuclear Factor {kappa}b Activation. JEM 191: 1735-1744 [Abstract] [Full Text]
Satterthwaite, A. B., Lowell, C. A., Khan, W. N., Sideras, P., Alt, F. W., Witte, O. N. (1998). Independent and Opposing Roles For Btk and Lyn in B and Myeloid Signaling Pathways. JEM 188: 833-844 [Abstract] [Full Text]
Satterthwaite, A. B., Cheroutre, H., Khan, W. N., Sideras, P., Witte, O. N. (1997). Btk dosage determines sensitivity to B cell antigen receptor�cross-linking. Proc. Natl. Acad. Sci. USA 94: 13152-13157 [Abstract] [Full Text]
Thomas, J., Sideras, P, Smith, C., Vorechovsky, I, Chapman, V, Paul, W. (1993). Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes. Science 261: 355-358 [Abstract]
Fruman, D. A., Ferl, G. Z., An, S. S., Donahue, A. C., Satterthwaite, A. B., Witte, O. N. (2002). Phosphoinositide 3-kinase and Bruton's tyrosine kinase regulate overlapping sets of genes in B lymphocytes. Proc. Natl. Acad. Sci. USA 99: 359-364 [Abstract] [Full Text]