Journal of Experimental Medicine, Vol 150, 1432-1447, Copyright © 1979 by Rockefeller University Press
Soluble factors in tolerance and contact sensitivity to 2,4- dinitrofluorobenzene in mice. III. Histocompatibility antigens associated with the hapten dinitrophenol serve as target molecules on 2,4-dinitrofluorobenzene-immune T cells for soluble suppressor factor
JW Moorhead
Previous studies have shown that suppression of 2,4- dinitrofluorobenzene
(DNFB) contact sensitivity by soluble suppressor factor (SSF) requires that
the donor of immune lymph node (LN) cells and of SSF share either the H-2K
and/or H-2D region of the major histocompatibility complex. Thus, target or
acceptor molecules for SSF appear to be coded for by genes within the H-2K
and H-2D loci. Experiments were done to investigate the nature of these
target molecules and to determine what cell types expressed them. It was
found that purified lymph node T cells are suppressed by SSF indicating
that T cells express the acceptor molecules. Adsorption experiments showed
that the only cells capable of adsorbing the suppressor factor are DNFB-
immune T cells from donors which share with the factor-producing strain
either the H-2K or H-2D locus. This adsorption can be specifically blocked
by pretreating the immune LN cells with antibodies directed against H-2K
and/or H-2D determinants or against the hapten DNP but not by antibodies
against Ia or theta-antigens. Collectively, these results indicate that the
target molecules are expressed only by DNFB-immune T cells and are
comprised of histocompatibility antigens associated with DNP.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
