The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 150, 698-702, Copyright © 1979 by Rockefeller University Press

ARTICLES

Generation of anti-type III pneumococcal polysaccharide hybridomas from mice with an X-linked B-lymphocyte defect

KR Schroer, KJ Kim, B Prescott and PJ Baker

(CBA/N X BALB/c male)F1 mice bear on X-linked defect making them totally unresponsive to T-independent (TI), TI-2 antigens such as type III pneumococcal polysaccharide (SSS-III). We found that somatic cell hybrids between CB nonresponder spleen cells and NS1 plasmacytoma cells secreted antibody specific for SSS-III. The solid-phase binding of such antibody was completely inhibited by the addition of free antigen (SSS- III) and the amount of antibody detected in culture fluids ranged from 10 ng/ml to 10 micrograms/ml. Eight hybridoma clones were identified; all make antibody of the IgM class. These results indicate that the X- linked defect does not result in a deletion of a B-cell subset which responds to TI-2 antigens.
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