Journal of Experimental Medicine, Vol 150, 507-516, Copyright © 1979 by Rockefeller University Press
Suppression of IgE antibody production in SJL mice. IV. Interaction of primed and unprimed T cells
T Itaya and Z Ovary
The mechanism of selective anti-hapten IgE antibody production was studied
in SJL mice. Using an adoptive transfer method of spleen cells into
syngeneic recipients irradiated with a sublethal dose of 600 rads, it was
demonstrated that for the suppression of anti-dinitrophenyl (DNP) IgE
antibody production the interaction of two subsets of T cells is necessary.
DNP-primed B cells and carrier-primed T helper cells are taken from donors
primed with small amounts of DNP-carrier conjugates. Without injection of
other cells, high titer and persistent anti-DNP antibodies are produced in
the recipients. The two subsets of T cells that are active in suppression
of IgE are taken from two types of donors: one donor is immunized
(hyperprimed) with larger amounts of carrier protein twice, the other is an
unprimed donor. The carrier for hyperpriming the first type of donor may be
unrelated to the carrier used for priming the helper T cells. To bring
about anti-DNP IgE suppression it is necessary that the animals should be
challenged with the same DNP-carrier conjugate used for priming the B and T
helper cells. If the hyperprimed donors were immunized with a heterologous,
unrelated carrier, then this heterologous unconjugated carrier must also be
injected together with the homologous DNP-carrier conjugate. In these
conditions, anti-DNP IgE antibody production is suppressed, but the
production of anti-DNP IgG1 antibody is not diminished.
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