Hyaluronidase-sensitive halos around adherent cells. Their role in blocking lymphocyte-mediated cytolysis

doi:10.1084/jem.149.2.507

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Hyaluronidase-sensitive halos around adherent cells. Their role in blocking lymphocyte-mediated cytolysis

WH McBride and JB Bard

A variety of adherent sarcoma, carcinoma and normal cells are surrounded in vitro by thick, transparent zones (approximately equal to 9 micron thick) that spleen cells and a variety of other cells and particles cannot penetrate. Seven lymphoblastoid cell lines did not possess such halos. The presence of these halos around adherent fibrosarcoma cells appeared to protect them from lymphocyte-mediated cytolysis. Hyaluronidase treatment, which destroyed the halo and allowed lymphocytes to approach the tumor cell membrane, enhanced the cytotoxic action of immune but not of normal spleen cells. These observations, in addition to highlighting a little-known feature of the cell surface, may also be of general relevance to the in vitro and in vivo killing of tumor cells by immune effector cells.
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del Fresno, C., Otero, K., Gomez-Garcia, L., Gonzalez-Leon, M. C., Soler-Ranger, L., Fuentes-Prior, P., Escoll, P., Baos, R., Caveda, L., Garcia, F., Arnalich, F., Lopez-Collazo, E. (2005). Tumor Cells Deactivate Human Monocytes by Up-Regulating IL-1 Receptor Associated Kinase-M Expression via CD44 and TLR4. J. Immunol. 174: 3032-3040 [Abstract] [Full Text]
Stern, R. (2003). Devising a pathway for hyaluronan catabolism: are we there yet?. Glycobiology 13: 105R-115R [Abstract] [Full Text]
Anttila, M. A., Tammi, R. H., Tammi, M. I., Syrjänen, K. J., Saarikoski, S. V., Kosma, V.-M. (2000). High Levels of Stromal Hyaluronan Predict Poor Disease Outcome in Epithelial Ovarian Cancer. Cancer Res. 60: 150-155 [Abstract] [Full Text]
Itano, N., Sawai, T., Miyaishi, O., Kimata, K. (1999). Relationship between Hyaluronan Production and Metastatic Potential of Mouse Mammary Carcinoma Cells. Cancer Res. 59: 2499-2504 [Abstract] [Full Text]
and Erik Fries, A. B. H., Blom, A. (1995). Inter-[IMAGE]-inhibitor Is Required for the Formation of the Hyaluronan-containing Coat on Fibroblasts and Mesothelial Cells. J. Biol. Chem. 270: 9698-9701 [Abstract] [Full Text]
Esko, J., Rostand, K., Weinke, J. (1988). Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096 [Abstract]
Dick, S., Macchi, B, Papazoglou, S, Oldfield, E., Kornblith, P., Smith, B., Gately, M. (1983). Lymphoid cell-glioma cell interaction enhances cell coat production by human gliomas: novel suppressor mechanism. Science 220: 739-742 [Abstract]