Journal of Experimental Medicine, Vol 148, 1510-1522, Copyright © 1978 by Rockefeller University Press

ARTICLES

The role of H-2 linked genes in helper T-cell function. IV. Importance of T-cell genotype and host environment in I-region and Ir gene expression

JW Kappler and P Marrack

We have studied the properties of helper T cells specific for sheep erythrocytes (SRBC), keyhole limpet hemocyanin (KLH), or poly-L- (Tyr,Glu)-poly-DL-Ala-poly-L-Lys [(T,G)-A--L]. These T cells differentiated and were primed in vivo in irradiation chimeras constructed of various combinations of F1 and parental bone marrow donors and irradiated recipients. Primed T cells were then tested for helper activity in the in vitro response of B cells and macrophages (Mphi) of parental or F1 origin to the hapten trinitrophenol coupled to the priming antigen. When testing either SRBC or KLH-specific T cells of parental H-2 type which had differentiated in F1 hosts, we found that they cooperated equally well with B cells and Mphi of either parental H-2 type. On the other hand, when testing F1 T cells which had differentiated in parental hosts, we found that they cooperated well only with B cells and Mphi which had the K-IA region type of the parental host. In similar experiments we found that (T,G)-A--L-specific T cells of low responder H-2 type which had differentiated in (high responder X low responder) F1 hosts induced high responses in high responder B cells and Mphi (T,G)-A--L-specific F1 T cells which differentiated in high responder but not those which differentiated in low responder hosts induced high responses in high responder B cells and Mphi. Low responder B cells and Mphi yielded low responses in all cases regardless of the source of (T,G)-A--L-specific T cells with what they were tested. Our results support the conclusion that I-region and Ir genes function via their expression in B cells and Mphi and in the host environment during helper T-cell differentiation, but not, at least under the conditions of these experiments, via their expression in the helper T cell itself. These findings place constraints upon models which attempt to explain the apparent dual recognition of antigen and I-region gene products by helper T cells.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Ku, C.-C., Kappler, J., Marrack, P. (2001). The Growth of the Very Large CD8+ T Cell Clones in Older Mice Is Controlled by Cytokines. J. Immunol. 166: 2186-2193 [Abstract] [Full Text]  
• Colson, Y. L., Tripp, R. A., Doherty, P. C., Wren, S. M., Neipp, M., Abou El-Ezz, A. Y., Ildstad, S. T. (1998). Antiviral Cytotoxic Activity Across a Species Barrier in Mixed Xenogeneic Chimeras: Functional Restriction to Host MHC. J. Immunol. 160: 3790-3796 [Abstract] [Full Text]  
• Ramsdell, F, Lantz, T, Fowlkes, B. (1989). A nondeletional mechanism of thymic self tolerance. Science 246: 1038-1041 [Abstract]  
• Blackman, M., Marrack, P, Kappler, J (1989). Influence of the major histocompatibility complex on positive thymic selection of V beta 17a+ T cells. Science 244: 214-217 [Abstract]  
• von Boehmer, H., Kishi, H., Borgulya, P., Scott, B., van Ewijk, W., Teh, H.S., Kisielow, P. (1989). Control of T-cell Development by the TCR{alpha}{beta} for Antigen. Cold Spring Harb Symp Quant Biol 54: 111-118 [Abstract]  
• Davis, M.M., Berg, L.J., Lin, A.Y., Fazekas de St. Groth, B., Devaux, B., Sagerstrom, C.G., Bjorkman, P.J., Elliott, J.F. (1989). TCR Recognition and Selection In Vivo. Cold Spring Harb Symp Quant Biol 54: 119-128 [Abstract]  
• Benacerraf, B (1981). Role of MHC gene products in immune regulation. Science 212: 1229-1238  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS