Journal of Experimental Medicine, Vol 147, 340-351, Copyright © 1978 by Rockefeller University Press

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Nonrandom inclusion of H-2K and H-2D antigens in Friend virus particles from mice of various strains

JE Bubbers, S Chen and F Lilly

Friend murine leukemia virus (FV), isolated from infectious serum of several mouse strains, has been examined for the presence of H-2 antigens. Following banding of the virus on a discontinuous sucrose gradient, pelleting, and disruption with Nonidet P-40 detergent, virus preparations were tested for their capacity to inhibit the lysis of target cells mediated by various anti-H-2K or anti-H-2D antisera. Virus from mice homozygous for the H-2b, H-2d, H-2g H-2k, and H-2ol haplotypes or heterozygous for the H-2g/H-2k, H-2b/H-2d, and H-2b/H-2k haplotypes was used. Of the six H-2D or H-2K alleles examined, the products of only two, H-2Db and H-2Kk were detected. Virus preparations contained no, one, or both antigens, depending on the genotype of the host animal. Control preparations from normal mouse serum and preparations in which the virus had not been disrupted demonstrated no H-2 activity. Furthermore, attempts to neutralize FV spleen focus forming activity with anti-H-2Db or anti-H-2Kk antisera yielded negative results.
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• Persson, H., Kvist, S., Ostberg, L., Peterson, P. A., Philipson, L. (1980). Adenoviral Early Glycoprotein E3-19K and Its Association with Transplantation Antigens. Cold Spring Harb Symp Quant Biol 44: 509-517 [Abstract]  

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