Journal of Experimental Medicine, Vol 146, 1748-1764,
Copyright © 1977 by Rockefeller University Press
The role of H-2-linked genes in helper T-cell function. I. In vitro expression in B cells of immune response genes controlling helper T-cell activity
JW Kappler
and
P Marrack
The ability of murine helper T cells primed to the antigen, sheep erythrocytes (SRBC) to cross-react with burro erythrocytes (BRBC) in the in vitro anti-trinitrophenol (TNP) response to TNP-RBC was shown to be under genetic control. Although non-H-2 genes were shown to influence the absolute level of helper activity assayed after SRBC priming, the extent of cross-reaction of SRBC-primed helpers with BRBC was shown to be controlled by an H-2-1inked Ir gene(s). H-2 haplotypes were identified which determined high, intermediate, or low response to the cross- reacting determinants and the gene(s) controlling the cross-reaction tentatively mapped to the K through I-E end of the H-2 complex. Helpers primed in F(1) mice of high x intermediate or high x low responder parents were tested for cross-reaction using B cells and macrophages (M
) of parental haplotypes. In each case the extent of cross-reaction was predicted by the H-2 haplotype of the B cells and M, establishing the expression of the Ir gene(s) in B cells and/or M a t least, but not ruling out its expression in T cells as well. The low cross-reaction seen when T cells from F(1) mice of high × low responder parents were tested on low responder B cells and M was not increased by the presence of high responder M, indicating the Ir gene(s) is expressed in the B cell a t least although it may be expressed in M as well. These and our previously reported experiments are consistent with the hypothesis that helper T cells recognize antigen bound to the surface of B cells and M in association with the product(s) of Ir gene(s) expressed on the B cell and M.
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