Journal of Experimental Medicine, Vol 146, 1603-1612, Copyright © 1977 by Rockefeller University Press
The genetic control of the immune response to staphylococcal nuclease VI. Recombination between genes determining the A/J anti-nuclease idiotypes and the heavy chain allotype locus
DS Pisetsky and DH Sachs
Rat antisera detecting binding site-specific idiotypic determinants on
anti-nuclease antibodies from A/J mice have been used to define the A/J
anti-nuclease idiotype and to investigate its genetic linkage as a variable
region marker. Analysis of the segregation of the A/J idiotype in progeny
of the backcross (B10.A X A/J) X B10.A showed linkage of the idiotype to
the Ig-1e heavy chain allotype locus. There was, however, a very high
apparent frequency of recombination, with 7 of 101 backcross animals having
a recombinant phenotype. All of these putative recombinants were accounted
for by Ig-1b/Ig-1b homozygotes which bore the A/J idiotype, and none by
Ig-1b/Ig-1e heterozygotes lacking the idiotype. On progeny testing of these
animals in another backcross to B10.A the recombinant trait bred true. If
this idiotype is indeed a marker for variable region structural genes, then
the germ line gene pool must be very large or there must be special genetic
mechanism to account for the increased recombinational frequency observed.
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