Induction of specific tissue transplantation tolerance using fractionated total lymphoid irradiation in adult mice: long-term survival of allogeneic bone marrow and skin grafts

doi:10.1084/jem.146.1.34

This Article

	Full Text (PDF, 1232K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Slavin, S.
	Articles by Kaplan, H. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Slavin, S.
	Articles by Kaplan, H. S.


Social Bookmarking

	What's this?

ARTICLES

Induction of specific tissue transplantation tolerance using fractionated total lymphoid irradiation in adult mice: long-term survival of allogeneic bone marrow and skin grafts

S Slavin, S Strober, Z Fuks and HS Kaplan

BALB/c mice were treated with fractionated high dose (3,400 rads) total lymphoid irradiation (TLI), and given semiallogeneic (BALB/c x C57BL/Ka) or allogeneic (C57BL/Ka) bone marrow and/or skin allografts. TLI alone prolonged the mean survival time (m.s.t.) of C57BL/Ka skin grafts to 49.1 days (control, 10.7 days). Shielding of the thymus during TLI produced only a slight increase in graft survival (m.s.t., 19 days). TLI combined with splenectomy was no more effective than TLI alone. Infusion of 10(7) semiallogeneic or allogeneic bone marrow cells after TLI produced stable chimeras in 7/8 and 8/15 recipients, respectively. Chimeras were specifically tolerant to donor tissues, since C57BL/Ka skin grafts were accepted for more than 250 days, but third-party (C3H/He) skin grafts were rejected rapidly. In addition, chimeric lymphocytes responded to C3H/He and C3H. Q but not to C57BL/Ka cells in the one-way mixed leukocyte reactions. BALB/c C57BL/Ka chimeras showed no clinical evidence of graft vs. host disease. These findings may have application of clinical organ transplantation, since (a) the recipient treatment (TLI) has already been shown to be safe in humans, (b) donors and recipients can be completely allogeneic, and (c) bone marrow and skin graft survival was permanent (greater than 250 days).
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Nusair, S., Or, R., Junadi, S., Amir, G., Breuer, R. (2005). Simultaneous Donor Marrow Cell Transplantation With Reduced Intensity Conditioning Prevents Tracheal Allograft Obliteration in a Bronchiolitis Obliterans Murine Model. Chest 128: 4024-4029 [Abstract] [Full Text]
Yung, I., Weiss, L., Abdul-Hai, A., Kasir, J., Reich, S., Slavin, S. (2005). Induction of Early Post-Transplant Graft-versus-Leukemia Effects Using Intentionally Mismatched Donor Lymphocytes and Elimination of Alloantigen-Primed Donor Lymphocytes for Prevention of Graft-versus-Host Disease. Cancer Res. 65: 9735-9740 [Abstract] [Full Text]
Tian, C., Bagley, J., Forman, D., Iacomini, J. (2004). Induction of Central Tolerance by Mature T Cells. J. Immunol. 173: 7217-7222 [Abstract] [Full Text]
Colson, Y. L., Xu, H., Huang, Y., Ildstad, S. T. (2004). Mixed Xenogeneic Chimerism Induces Donor-Specific Humoral and Cellular Immune Tolerance for Cardiac Xenografts. J. Immunol. 173: 5827-5834 [Abstract] [Full Text]
Williams, M. A., Adams, A. B., Walsh, M. B., Shirasugi, N., Onami, T. M., Pearson, T. C., Ahmed, R., Larsen, C. P. (2003). Primary and Secondary Immunocompetence in Mixed Allogeneic Chimeras. J. Immunol. 170: 2382-2389 [Abstract] [Full Text]
Rigby, S. M., Rouse, T., Field, E. H. (2003). Total lymphoid irradiation nonmyeloablative preconditioning enriches for IL-4-producing CD4+-TNK cells and skews differentiation of immunocompetent donor CD4+ cells. Blood 101: 2024-2032 [Abstract] [Full Text]
Higuchi, M., Zeng, D., Shizuru, J., Gworek, J., Dejbakhsh-Jones, S., Taniguchi, M., Strober, S. (2002). Immune Tolerance to Combined Organ and Bone Marrow Transplants After Fractionated Lymphoid Irradiation Involves Regulatory NK T Cells and Clonal Deletion. J. Immunol. 169: 5564-5570 [Abstract] [Full Text]
Eto, M., Hackstein, H., Kaneko, K., Nomoto, K., Thomson, A. W. (2002). Promotion of Skin Graft Tolerance Across MHC Barriers by Mobilization of Dendritic Cells in Donor Hemopoietic Cell Infusions. J. Immunol. 169: 2390-2396 [Abstract] [Full Text]
Xu, H., Exner, B. G., Cramer, D. E., Tanner, M. K., Mueller, Y. M., Ildstad, S. T. (2002). CD8+, {alpha}{beta}-TCR+, and {gamma}{delta}-TCR+ Cells in the Recipient Hematopoietic Environment Mediate Resistance to Engraftment of Allogeneic Donor Bone Marrow. J. Immunol. 168: 1636-1643 [Abstract] [Full Text]
Adams, A. B., Durham, M. M., Kean, L., Shirasugi, N., Ha, J., Williams, M. A., Rees, P. A., Cheung, M. C., Mittelstaedt, S., Bingaman, A. W., Archer, D. R., Pearson, T. C., Waller, E. K., Larsen, C. P. (2001). Costimulation Blockade, Busulfan, and Bone Marrow Promote Titratable Macrochimerism, Induce Transplantation Tolerance, and Correct Genetic Hemoglobinopathies with Minimal Myelosuppression. J. Immunol. 167: 1103-1111 [Abstract] [Full Text]
Nikolic, B., Zhao, G., Swenson, K., Sykes, M. (2000). A novel application of cyclosporine A in nonmyeloablative pretransplant host conditioning for allogeneic BMT. Blood 96: 1166-1172 [Abstract] [Full Text]
Pham, S. M., Rao, A. S., Zeevi, A., Kormos, R. L., McCurry, K. R., Hattler, B. G., Fung, J. J., Starzl, T. E., Griffith, B. P. (2000). A CLINICAL TRIAL COMBINING DONOR BONE MARROW INFUSION AND HEART TRANSPLANTATION: INTERMEDIATE-TERM RESULTS. J. Thorac. Cardiovasc. Surg. 119: 673-681 [Abstract] [Full Text]
Pham, S. M., Rao, A. S., Zeevi, A., McCurry, K. R., Keenan, R. J., Vega, J. D., Kormos, R. L., Hattler, B. G., Fung, J. J., Starzl, T. E., Griffith, B. P. (2000). Effects of donor bone marrow infusion in clinical lung transplantation. Ann. Thorac. Surg. 69: 345-350 [Abstract] [Full Text]
Storb, R., Yu, C., Barnett, T., Wagner, J. L., Deeg, H. J., Nash, R. A., Kiem, H.-P., McSweeney, P., Seidel, K., Georges, G., Zaucha, J. M. (1999). Stable Mixed Hematopoietic Chimerism in Dog Leukocyte Antigen-Identical Littermate Dogs Given Lymph Node Irradiation Before and Pharmacologic Immunosuppression After Marrow Transplantation. Blood 94: 1131-1136 [Abstract] [Full Text]
Slavin, S., Nagler, A., Naparstek, E., Kapelushnik, Y., Aker, M., Cividalli, G., Varadi, G., Kirschbaum, M., Ackerstein, A., Samuel, S., Amar, A., Brautbar, C., Ben-Tal, O., Eldor, A., Or, R. (1998). Nonmyeloablative Stem Cell Transplantation and Cell Therapy as an Alternative to Conventional Bone Marrow Transplantation With Lethal Cytoreduction for the Treatment of Malignant and Nonmalignant Hematologic Diseases. Blood 91: 756-763 [Abstract] [Full Text]
Pham, S. M., Keenan, R. J., Rao, A. S., Fontes, P. A., Kormos, R. L., Abu-Elmagd, K., Zeevi, A., Kawai, A., Hattler, B. G., Hardesty, R. L., Demetris, A. J., Trucco, M. M., Rosner, G. L., Fung, J. J., Starzl, T. E., Griffith, B. P. (1995). Perioperative Donor Bone Marrow Infusion Augments Chimerism in Heart and Lung Transplant Recipients. Ann. Thorac. Surg. 60: 1015-1020 [Abstract] [Full Text]
Waldmann, H., Cobbold, S.P., Qin, S., Benjamin, R.J., Wise, M. (1989). Tolerance Induction in the Adult Using Monoclonal Antibodies to CD4, CD8, and CD11a (LFA-1). Cold Spring Harb Symp Quant Biol 54: 885-892 [Abstract]
BEN-CHETRIT, E., GROSS, D. J., BRAVERMAN, A., WESHLER, Z., FUKS, Z., SLAVIN, S., ELIAKIM, M. (1986). Total Lymphoid Irradiation in Refractory Systemic Lupus Erythematosus. ANN INTERN MED 105: 58-60 [Abstract]
GALE, R. P., CHAMPLIN, R. E., FEIG, S. A., FITCHEN, J. H. (1981). Aplastic Anemia: Biology and Treatment. ANN INTERN MED 95: 477-494 [Abstract]
Slavin, S, Yatziv, S (1980). Correction of enzyme deficiency in mice by allogeneic bone marrow transplantation with total lymphoid irradiation. Science 210: 1150-1152 [Abstract]