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Cultured mononuclear peripheral blood leukocytes (PBL) from nonimmune human beings and monkeys are nonpermissive to dengue 2 virus (D2V) infection at multiplicities of infection of 0.001-0.1, but become permissive when non-neutralizing dengue antibody is added to medium. D2V infection occurred in PBL prepared from anti-coagulated but not from defibrinated plasma. Infection enhancement was produced by multiple lots of heterotypic anti-dengue raised in several mammalian species. Homotypic anti-dengue neutralized D2V at high concentrations but enhanced at low concentrations; enhancement end point in one serum was 1:320,000. The infection-enhancing factor was a noncytophilic antibody of the IgG class. D2V infection occurred in the absence of heat-labile complement components but did not occur when complexes were prepared with anti- dengue F(ab)(2). Treatment of PBL with several proteases increased permissiveness to D2V infection by immune complexes but not by virus alone. Two rhesus monkey serums collected 14 days after D2V infection contained an IgG antibody with high-titered enhancing activity but with no hemagglutination-inhibition or neutralizing activity. Virus-antibody complexes are irreversibly attached to PBL within 15 min and completely internalized in 60 min. There was considerable variation in cellular infection in different experiments, however, maximum virus yields usually exceeded 1,000 plaque-forming units per 1 x 10(6) PBL occurring between 2 and 4 days in culture. In vitro antibody-dependent infection of PBL provides a possible model for study of pathogenetic mechanisms in infants with dengue shock syndrome who passively acquire maternal anti-dengue IgG.
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• Blackley, S., Kou, Z., Chen, H., Quinn, M., Rose, R. C., Schlesinger, J. J., Coppage, M., Jin, X. (2007). Primary Human Splenic Macrophages, but Not T or B Cells, Are the Principal Target Cells for Dengue Virus Infection In Vitro. J. Virol. 81: 13325-13334 [Abstract] [Full Text]  
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• Mott, K. R., Osorio, Y., Maguen, E., Nesburn, A. B., Wittek, A. E., Cai, S., Chattopadhyay, S., Ghiasi, H. (2007). Role of Anti-Glycoproteins D (Anti-gD) and K (Anti-gK) IgGs in Pathology of Herpes Stromal Keratitis in Humans. IOVS 48: 2185-2193 [Abstract] [Full Text]  
• Srikiatkhachorn, A., Ajariyakhajorn, C., Endy, T. P., Kalayanarooj, S., Libraty, D. H., Green, S., Ennis, F. A., Rothman, A. L. (2007). Virus-Induced Decline in Soluble Vascular Endothelial Growth Receptor 2 Is Associated with Plasma Leakage in Dengue Hemorrhagic Fever. J. Virol. 81: 1592-1600 [Abstract] [Full Text]  
• Lee, Y.-R., Liu, M.-T., Lei, H.-Y., Liu, C.-C., Wu, J.-M., Tung, Y.-C., Lin, Y.-S., Yeh, T.-M., Chen, S.-H., Liu, H.-S. (2006). MCP-1, a highly expressed chemokine in dengue haemorrhagic fever/dengue shock syndrome patients, may cause permeability change, possibly through reduced tight junctions of vascular endothelium cells. J. Gen. Virol. 87: 3623-3630 [Abstract] [Full Text]  
• Sun, P., Celluzzi, C. M., Marovich, M., Subramanian, H., Eller, M., Widjaja, S., Palmer, D., Porter, K., Sun, W., Burgess, T. (2006). CD40 Ligand Enhances Dengue Viral Infection of Dendritic Cells: A Possible Mechanism for T Cell-Mediated Immunopathology. J. Immunol. 177: 6497-6503 [Abstract] [Full Text]  
• Mongkolsapaya, J., Duangchinda, T., Dejnirattisai, W., Vasanawathana, S., Avirutnan, P., Jairungsri, A., Khemnu, N., Tangthawornchaikul, N., Chotiyarnwong, P., Sae-Jang, K., Koch, M., Jones, Y., McMichael, A., Xu, X., Malasit, P., Screaton, G. (2006). T Cell Responses in Dengue Hemorrhagic Fever: Are Cross-Reactive T Cells Suboptimal?. J. Immunol. 176: 3821-3829 [Abstract] [Full Text]  
• Calvert, A. E., Huang, C. Y.-H., Kinney, R. M., Roehrig, J. T. (2006). Non-structural proteins of dengue 2 virus offer limited protection to interferon-deficient mice after dengue 2 virus challenge. J. Gen. Virol. 87: 339-346 [Abstract] [Full Text]  
• CHEN, L.-C., LEI, H.-Y., LIU, C.-C., SHIESH, S.-C., CHEN, S.-H., LIU, H.-S., LIN, Y.-S., WANG, S.-T., SHYU, H.-W., YEH, T.-M. (2006). CORRELATION OF SERUM LEVELS OF MACROPHAGE MIGRATION INHIBITORY FACTOR WITH DISEASE SEVERITY AND CLINICAL OUTCOME IN DENGUE PATIENTS. Am J Trop Med Hyg 74: 142-147 [Abstract] [Full Text]  
• Matheus, S., Deparis, X., Labeau, B., Lelarge, J., Morvan, J., Dussart, P. (2005). Use of Four Dengue Virus Antigens for Determination of Dengue Immune Status by Enzyme-Linked Immunosorbent Assay of Immunoglobulin G Avidity. J. Clin. Microbiol. 43: 5784-5786 [Abstract] [Full Text]  
• Wilder-Smith, A., Schwartz, E. (2005). Dengue in Travelers. NEJM 353: 924-932 [Full Text]  
• Matheus, S., Deparis, X., Labeau, B., Lelarge, J., Morvan, J., Dussart, P. (2005). Discrimination between Primary and Secondary Dengue Virus Infection by an Immunoglobulin G Avidity Test Using a Single Acute-Phase Serum Sample. J. Clin. Microbiol. 43: 2793-2797 [Abstract] [Full Text]  
• Simmons, C. P., Dong, T., Chau, N. V., Dung, N. T. P., Chau, T. N. B., Thao, L. T. T., Dung, N. T., Hien, T. T., Rowland-Jones, S., Farrar, J. (2005). Early T-Cell Responses to Dengue Virus Epitopes in Vietnamese Adults with Secondary Dengue Virus Infections. J. Virol. 79: 5665-5675 [Abstract] [Full Text]  
• Modis, Y., Ogata, S., Clements, D., Harrison, S. C. (2005). Variable Surface Epitopes in the Crystal Structure of Dengue Virus Type 3 Envelope Glycoprotein. J. Virol. 79: 1223-1231 [Abstract] [Full Text]  
• de Souza, V. A. U. F., Fernandes, S., Araujo, E. S., Tateno, A. F., Oliveira, O. M. N. P. F., Oliveira, R. d. R., Pannuti, C. S. (2004). Use of an Immunoglobulin G Avidity Test To Discriminate between Primary and Secondary Dengue Virus Infections. J. Clin. Microbiol. 42: 1782-1784 [Abstract] [Full Text]  
• Warke, R. V., Xhaja, K., Martin, K. J., Fournier, M. F., Shaw, S. K., Brizuela, N., de Bosch, N., Lapointe, D., Ennis, F. A., Rothman, A. L., Bosch, I. (2003). Dengue Virus Induces Novel Changes in Gene Expression of Human Umbilical Vein Endothelial Cells. J. Virol. 77: 11822-11832 [Abstract] [Full Text]  
• Wallace, M. J., Smith, D. W., Broom, A. K., Mackenzie, J. S., Hall, R. A., Shellam, G. R., McMinn, P. C. (2003). Antibody-dependent enhancement of Murray Valley encephalitis virus virulence in mice. J. Gen. Virol. 84: 1723-1728 [Abstract] [Full Text]  
• Takada, A., Feldmann, H., Ksiazek, T. G., Kawaoka, Y. (2003). Antibody-Dependent Enhancement of Ebola Virus Infection. J. Virol. 77: 7539-7544 [Abstract] [Full Text]  
• Cologna, R., Rico-Hesse, R. (2003). American Genotype Structures Decrease Dengue Virus Output from Human Monocytes and Dendritic Cells. J. Virol. 77: 3929-3938 [Abstract] [Full Text]  
• Weiser, J. N., Bae, D., Fasching, C., Scamurra, R. W., Ratner, A. J., Janoff, E. N. (2003). Antibody-enhanced pneumococcal adherence requires IgA1 protease. Proc. Natl. Acad. Sci. USA 100: 4215-4220 [Abstract] [Full Text]  
• Lin, Y.-W., Wang, K.-J., Lei, H.-Y., Lin, Y.-S., Yeh, T.-M., Liu, H.-S., Liu, C.-C., Chen, S.-H. (2002). Virus Replication and Cytokine Production in Dengue Virus-Infected Human B Lymphocytes. J. Virol. 76: 12242-12249 [Abstract] [Full Text]  
• Chen, Y.-C., Wang, S.-Y. (2002). Activation of Terminally Differentiated Human Monocytes/Macrophages by Dengue Virus: Productive Infection, Hierarchical Production of Innate Cytokines and Chemokines, and the Synergistic Effect of Lipopolysaccharide. J. Virol. 76: 9877-9887 [Abstract] [Full Text]  
• King, C. A., Anderson, R., Marshall, J. S. (2002). Dengue Virus Selectively Induces Human Mast Cell Chemokine Production. J. Virol. 76: 8408-8419 [Abstract] [Full Text]  
• Wang, T., Anderson, J. F., Magnarelli, L. A., Wong, S. J., Koski, R. A., Fikrig, E. (2001). Immunization of Mice Against West Nile Virus with Recombinant Envelope Protein. J. Immunol. 167: 5273-5277 [Abstract] [Full Text]  
• Thullier, P., Demangel, C., Bedouelle, H., Megret, F., Jouan, A., Deubel, V., Mazie, J.-C., Lafaye, P. (2001). Mapping of a dengue virus neutralizing epitope critical for the infectivity of all serotypes: insight into the neutralization mechanism. J. Gen. Virol. 82: 1885-1892 [Abstract] [Full Text]  
• Takada, A., Watanabe, S., Okazaki, K., Kida, H., Kawaoka, Y. (2001). Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein. J. Virol. 75: 2324-2330 [Abstract] [Full Text]  
• Hahn, C. S., French, O. G., Foley, P., Martin, E. N., Taylor, R. P. (2001). Bispecific Monoclonal Antibodies Mediate Binding of Dengue Virus to Erythrocytes in a Monkey Model of Passive Viremia. J. Immunol. 166: 1057-1065 [Abstract] [Full Text]  
• Huang, K.-J., Li, S.-Y. J., Chen, S.-C., Liu, H.-S., Lin, Y.-S., Yeh, T.-M., Liu, C.-C., Lei, H.-Y. (2000). Manifestation of thrombocytopenia in dengue-2-virus-infected mice. J. Gen. Virol. 81: 2177-2182 [Abstract] [Full Text]  
• King, C. A., Marshall, J. S., Alshurafa, H., Anderson, R. (2000). Release of Vasoactive Cytokines by Antibody-Enhanced Dengue Virus Infection of a Human Mast Cell/Basophil Line. J. Virol. 74: 7146-7150 [Abstract] [Full Text]  
• Seiler, P., Senn, B. M., Klenerman, P., Kalinke, U., Hengartner, H., Zinkernagel, R. M. (2000). Additive Effect of Neutralizing Antibody and Antiviral Drug Treatment in Preventing Virus Escape and Persistence. J. Virol. 74: 5896-5901 [Abstract] [Full Text]  
• Cuzzubbo, A. J., Vaughn, D. W., Nisalak, A., Solomon, T., Kalayanarooj, S., Aaskov, J., Dung, N. M., Devine, P. L. (1999). Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia. CVI 6: 705-712 [Abstract] [Full Text]  
• Ferguson, N., Anderson, R., Gupta, S. (1999). The effect of antibody-dependent enhancement on the transmission dynamics and persistence of multiple-strain pathogens. Proc. Natl. Acad. Sci. USA 96: 790-794 [Abstract] [Full Text]  
• Lin, Y.-L., Liao, C.-L., Chen, L.-K., Yeh, C.-T., Liu, C.-I, Ma, S.-H., Huang, Y.-Y., Huang, Y.-L., Kao, C.-L., King, C.-C. (1998). Study of Dengue Virus Infection in SCID Mice Engrafted with Human K562 Cells. J. Virol. 72: 9729-9737 [Abstract] [Full Text]  
• Avirutnan, P., Malasit, P., Seliger, B., Bhakdi, S., Husmann, M. (1998). Dengue Virus Infection of Human Endothelial Cells Leads to Chemokine Production, Complement Activation, and Apoptosis. J. Immunol. 161: 6338-6346 [Abstract] [Full Text]  
• Seiler, P., Brundler, M.-A., Zimmermann, C., Weibel, D., Bruns, M., Hengartner, H., Zinkernagel, R. M. (1998). Induction of Protective Cytotoxic T Cell Responses in the Presence of High Titers of Virus-neutralizing Antibodies: Implications for Passive and Active Immunization. JEM 187: 649-654 [Abstract] [Full Text]  
• Haynes, B. (1993). Scientific and social issues of human immunodeficiency virus vaccine development. Science 260: 1279-1286 [Abstract]  
• Takeda, A, Tuazon, C., Ennis, F. (1988). Antibody-enhanced infection by HIV-1 via Fc receptor-mediated entry. Science 242: 580-583 [Abstract]  
• Koff, W., Hoth, D. (1988). Development and testing of AIDS vaccines. Science 241: 426-432 [Abstract]  
• Halstead, S. (1988). Pathogenesis of dengue: challenges to molecular biology. Science 239: 476-481 [Abstract]  

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