Journal of Experimental Medicine, Vol 145, 749-754, Copyright © 1977 by Rockefeller University Press

ARTICLES

DNA-binding property of Sm nuclear antigen

PA Reyes and EM Tan
Department of Clinical Research, Scripps Clinic and Research Foundation, La Jolla, California 92037.

Antibodies in the sera of patients with systemic lupus erythematosus reacted with a nuclear acidic protein called Sm antigen, and these antibodies were used as reagents to identify Sm antigen in preparative fractionation procedures. DNA affinity chromatography showed that Sm antigen was associated with nuclear protein fractions which had DNA- binding capacity. Evidence was also presented that Sm antigen showed preferential binding for single-strand DNA over double-strand DNA. These studies demonstrate that spontaneously occurring anti-nuclear antibodies in disease states may be used to study the properties of cellular proteins which are present in trace amounts.
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• Dieker, J W, Van Bavel, C C, Riemekasten, G, Berden, J H, van der Vlag, J (2006). The binding of lupus-derived autoantibodies to the C-terminal peptide (83-119) of the major SmD1 autoantigen can be mediated by double-stranded DNA and nucleosomes. Ann Rheum Dis 65: 1525-1528 [Abstract] [Full Text]  
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