Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors

doi:10.1084/jem.145.3.508

This Article

	Full Text (PDF, 1154K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lindahl, K. F.
	Articles by Wilson, D. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lindahl, K. F.
	Articles by Wilson, D. B.


Social Bookmarking

	What's this?

ARTICLES

Histocompatibility antigen-activated cytotoxic T lymphocytes. II. Estimates of the frequency and specificity of precursors

KF Lindahl and DB Wilson
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19174.

Using limiting dilutions of responding cells in mouse mixed leukocyte cultures, we obtained direct estimates of the minimum frequency of precursors of cytotoxic T lymphocytes (CTL.P) for a variety of antigens. Depending on the strain combination, there were as many as 4- 15 CTL.P reactive to DBA/2 among 10(4) lymph node cells. Taking into account that only 5-10% of peripheral T lymphocytes have the potential to develop into cytotoxic T lymphocytes (CTLs) (6), this implies that at least 1-2% of all CTL.P are responsive to any given H-2 haplotype difference. Precursors of cytotoxic cells thus have the same high frequency of cells reactive to alloantigens of the major histocompatibility complex as found among proliferating cells in graft- vs.-host reactions and mixed lymphocyte interactions. The frequencies of CTL.P reactive to xenoantigens (rat) or trinitrophenyl-modified self were less than half the frequency of alloreactive CTL.P. A minority of the CTL.P specific for one H-2 haplotype were also reactive to a third party H-2 haplotype, presumably on the basis of recognition of shared determinants. By dilution of sensitized cells from single microcultures, it was shown that a single CTL.P undergoes a minimum of three to four cell divisions and generates at least 8-16 CTLs after antigenic activation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Kumar, P., Vahedi-Faridi, A., Saenger, W., Merino, E., Lopez de Castro, J. A., Uchanska-Ziegler, B., Ziegler, A. (2009). Structural Basis for T Cell Alloreactivity among Three HLA-B14 and HLA-B27 Antigens. J. Biol. Chem. 284: 29784-29797 [Abstract] [Full Text]
Ishizuka, J., Grebe, K., Shenderov, E., Peters, B., Chen, Q., Peng, Y., Wang, L., Dong, T., Pasquetto, V., Oseroff, C., Sidney, J., Hickman, H., Cerundolo, V., Sette, A., Bennink, J. R., McMichael, A., Yewdell, J. W. (2009). Quantitating T Cell Cross-Reactivity for Unrelated Peptide Antigens. J. Immunol. 183: 4337-4345 [Abstract] [Full Text]
Griffith, B. P., Haddad, M., Poston, R. S. (2008). Immunobiology of Heart and Heart-Lung Transplantation. Card Surg Adult 3: 1513-1538 [Full Text]
Selin, L. K., Brehm, M. A. (2007). Frontiers in Nephrology: Heterologous Immunity, T Cell Cross-Reactivity, and Alloreactivity. J. Am. Soc. Nephrol. 18: 2268-2277 [Abstract] [Full Text]
Wang, Z., Davies, J. D. (2007). CD8 Blockade Promotes Antigen Responsiveness to Nontolerizing Antigen in Tolerant Mice by Inhibiting Apoptosis of CD4+ T Cells. J. Immunol. 178: 6148-6157 [Abstract] [Full Text]
AbuAttieh, M., Rebrovich, M., Wettstein, P. J., Vuk-Pavlovic, Z., Limper, A. H., Platt, J. L., Cascalho, M. (2007). Fitness of Cell-Mediated Immunity Independent of Repertoire Diversity. J. Immunol. 178: 2950-2960 [Abstract] [Full Text]
Brehm, M. A., Mangada, J., Markees, T. G., Pearson, T., Daniels, K. A., Thornley, T. B., Welsh, R. M., Rossini, A. A., Greiner, D. L. (2007). Rapid quantification of naive alloreactive T cells by TNF-{alpha} production and correlation with allograft rejection in mice. Blood 109: 819-826 [Abstract] [Full Text]
Landais, E., Morice, A., Long, H. M., Haigh, T. A., Charreau, B., Bonneville, M., Taylor, G. S., Houssaint, E. (2006). EBV-Specific CD4+ T Cell Clones Exhibit Vigorous Allogeneic Responses. J. Immunol. 177: 1427-1433 [Abstract] [Full Text]
Mannon, R. B., Kirk, A. D. (2006). Beyond Histology: Novel Tools to Diagnose Allograft Dysfunction. CJASN 1: 358-366 [Abstract] [Full Text]
Koehn, B., Gangappa, S., Miller, J. D., Ahmed, R., Larsen, C. P. (2006). Patients, pathogens, and protective immunity: the relevance of virus-induced alloreactivity in transplantation.. J. Immunol. 176: 2691-2696 [Abstract] [Full Text]
Brehm, M. A., Daniels, K. A., Ortaldo, J. R., Welsh, R. M. (2005). Rapid Conversion of Effector Mechanisms from NK to T Cells during Virus-Induced Lysis of Allogeneic Implants In Vivo. J. Immunol. 174: 6663-6671 [Abstract] [Full Text]
Tesar, B. M., Chalasani, G., Smith-Diggs, L., Baddoura, F. K., Lakkis, F. G., Goldstein, D. R. (2004). Direct Antigen Presentation by a Xenograft Induces Immunity Independently of Secondary Lymphoid Organs. J. Immunol. 173: 4377-4386 [Abstract] [Full Text]
Takaki, T., Lieberman, S. M., Holl, T. M., Han, B., Santamaria, P., Serreze, D. V., DiLorenzo, T. P. (2004). Requirement for Both H-2Db and H-2Kd for the Induction of Diabetes by the Promiscuous CD8+ T Cell Clonotype AI4. J. Immunol. 173: 2530-2541 [Abstract] [Full Text]
Richards, D. M., Dalheimer, S. L., Ehst, B. D., Vanasek, T. L., Jenkins, M. K., Hertz, M. I., Mueller, D. L. (2004). Indirect Minor Histocompatibility Antigen Presentation by Allograft Recipient Cells in the Draining Lymph Node Leads to the Activation and Clonal Expansion of CD4+ T Cells That Cause Obliterative Airways Disease. J. Immunol. 172: 3469-3479 [Abstract] [Full Text]
Griffith, B. P., Poston, R. S. (2003). Immunobiology of Heart and Heart-Lung Transplantation. Card Surg Adult 2: 1403-1426 [Full Text]
Pantenburg, B., Heinzel, F., Das, L., Heeger, P. S., Valujskikh, A. (2002). T Cells Primed by Leishmania major Infection Cross-React with Alloantigens and Alter the Course of Allograft Rejection. J. Immunol. 169: 3686-3693 [Abstract] [Full Text]
Chiffoleau, E., Beriou, G., Dutartre, P., Usal, C., Soulillou, J.-P., Cuturi, M. C. (2002). Role for Thymic and Splenic Regulatory CD4+ T Cells Induced by Donor Dendritic Cells in Allograft Tolerance by LF15-0195 Treatment. J. Immunol. 168: 5058-5069 [Abstract] [Full Text]
Sebille, F., Gagne, K., Guillet, M., Degauque, N., Pallier, A., Brouard, S., Vanhove, B., Delsuc, M.-A., Soulillou, J.-P. (2001). Direct Recognition of Foreign MHC Determinants by Naive T Cells Mobilizes Specific V{beta} Families Without Skewing of the Complementarity-Determining Region 3 Length Distribution. J. Immunol. 167: 3082-3088 [Abstract] [Full Text]
Portugal, K., Dozmorov, I., Sidorov, I., Marrero, I., Fonseca, J. A., Spadafora-Ferreira, M., Kalil, J., Coelho, V. (2001). Renal transplant patients show variations in their self-reactive repertoires: a serial study. Int Immunol 13: 747-755 [Abstract] [Full Text]
Trop, S., Charron, J., Arguin, C., Hugo, P. (2000). Thymic Selection Generates T Cells Expressing Self-Reactive TCRs in the Absence of CD45. J. Immunol. 165: 3073-3079 [Abstract] [Full Text]
Bingaman, A. W., Ha, J., Waitze, S.-Y., Durham, M. M., Cho, H. R., Tucker-Burden, C., Hendrix, R., Cowan, S. R., Pearson, T. C., Larsen, C. P. (2000). Vigorous Allograft Rejection in the Absence of Danger. J. Immunol. 164: 3065-3071 [Abstract] [Full Text]
McKay, D. B., Irie, H. Y., Hollander, G., Ferrara, J. L. M. F., Strom, T. B., Li, Y., Burakoff, S. J. (1999). Antigen-Induced Unresponsiveness Results in Altered T Cell Signaling. J. Immunol. 163: 6455-6461 [Abstract] [Full Text]
Davies, J. D., O'Connor, E., Hall, D., Krahl, T., Trotter, J., Sarvetnick, N. (1999). CD4+ CD45RB Low-Density Cells from Untreated Mice Prevent Acute Allograft Rejection. J. Immunol. 163: 5353-5357 [Abstract] [Full Text]
Heeger, P. S., Greenspan, N. S., Kuhlenschmidt, S., Dejelo, C., Hricik, D. E., Schulak, J. A., Tary-Lehmann, M. (1999). Pretransplant Frequency of Donor-Specific, IFN-{gamma}-Producing Lymphocytes Is a Manifestation of Immunologic Memory and Correlates with the Risk of Posttransplant Rejection Episodes. J. Immunol. 163: 2267-2275 [Abstract] [Full Text]
Munz, C., Obst, R., Osen, W., Stevanovic, S., Rammensee, H.-G. (1999). Alloreactivity as a Source of High Avidity Peptide-Specific Human CTL. J. Immunol. 162: 25-34 [Abstract] [Full Text]
Benichou, G., Valujskikh, A., Heeger, P. S. (1999). Contributions of Direct and Indirect T Cell Alloreactivity During Allograft Rejection in Mice. J. Immunol. 162: 352-358 [Abstract] [Full Text]
Matesic, D., Valujskikh, A., Pearlman, E., Higgins, A. W., Gilliam, A. C., Heeger, P. S. (1998). Type 2 Immune Deviation Has Differential Effects on Alloreactive CD4+ and CD8+ T Cells. J. Immunol. 161: 5236-5244 [Abstract] [Full Text]
Henderson, S. C., Berezovskaya, A., English, A., Palliser, D., Rock, K. L., Bamezai, A. (1998). CD4+ T Cells Mature in the Absence of MHC Class I and Class II Expression in Ly-6A.2 Transgenic Mice. J. Immunol. 161: 175-182 [Abstract] [Full Text]
Kusunoki, Y., Chen, W., Martin, P. J. (1998). Prevention of Marrow Graft Rejection Without Induction of Graft-Versus-Host Disease by a Cytotoxic T-Cell Clone That Recognizes Recipient Alloantigens. Blood 91: 4038-4044 [Abstract] [Full Text]