Journal of Experimental Medicine, Vol 145, 249-263, Copyright © 1977 by Rockefeller University Press
Defective colony formation by B lymphocytes from CBA/N and C3H/HeJ mice
PW Kincade
B cells from CBA/N mice did not form colonies in semisolid agar cultures
under circumstances where normal B-cell clonal proliferation is linear with
respect to the number of functional cells cultured. This was no due to the
unresponsiveness of CBA/N cells to mitogens, and under appropriate liquid
culture conditions many CBA/N lymphocytes differentiated to plasma cells
containing large amounts of IgM in response to LPS. On the other hand, the
same cells proliferated and matured poorly in liquid cultures prepared at
low-cell density. The frequency of granulocyte-macrophage progenitors and
multipotential hemopoietic stem cells in bone marrow, ability of peritoneal
macrophages to elaborate soluble enhancing factors, and levels of serum
inhibitors were normal in CBA/N mice. Together with the results of cell-
mixing experiments, these findings confirm the selective and intrinsic
nature of the CBA/N deficiency. It is suggested that the B-cell cloning
technique may be of value in selectively enumerating and assessing
functional capability of thymus-independent B cells. C3H/HeJ mice which
have previously only been known to be hyporesponsive to certain forms of
lipopolysaccharide had a subnormal incidence of colony-forming B cells.
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