Journal of Experimental Medicine, Vol 144, 617-626, Copyright © 1976 by Rockefeller University Press

ARTICLES

Helper function of T cells depleted of alloantigen-reactive lymphocytes by filtration through irradiated F1 hybrid recipients. I. Failure to collaborate with allogeneic B cells in a secondary response to sheep erythrocytes measured in vivo

J Sprent and H von Boehmer

Helper T cells were obtained by injecting heavily irradiated semiallogeneic mice with lymph node cells from H-2-incompatible parental strain mice primed with sheep erythrocytes (SRC) 2 mo before. Thoracic duct lymphocytes collected from the recipents 18-40 h later (nearly all of which were theta-positive and of donor origin) were totally and specifically unresponsive against host-type determinants in mixed-lymphocyte culture. The filtered cells were transferred to irradiated semiallogeneic mice together with SRC and anti-theta-serum- treated (B) cells from SRC-primed syngeneic, semiallogeneic, or allogeneic mice. When antibody-forming cells were measured in the spleen 5-9 days later, effective IgM and IgG collaborative responses were observed with both syngeneic and semiallogeneic B cells but not with allogeneic B cells. No evidence was found that the failure to obtain collaboration with the allogeneic B cells was due to inhibition of the B cells by the T cells or vice versa.
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This article has been cited by other articles:

• Paul, W., Benacerraf, B (1977). Functional specificity of thymus- dependent lymphocytes. Science 195: 1293-1300 [Abstract]  
• Boehmer, H. v., Sprent, J., Haas, W. (1977). The Influence of H-2 Gene Products on Cell-mediated and Humoral Immune Responses. Cold Spring Harb Symp Quant Biol 41: 539-545 [Abstract]  

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