Journal of Experimental Medicine, Vol 144, 98-112, Copyright © 1976 by Rockefeller University Press

ARTICLES

Isolation and immunologic characterization of a human. B-lymphocyte- specific, cell surface antigen

RE Humphreys, JM McCune, L Chess, HC Herrman, DJ Malenka, DL Mann, P Parham, SF Schlossman and JL Strominger

In addition to HL-A antigens, another cell surface protein complex has been obtained from membranes of the human B-lymphoblast cell line IM-1. This complex which was solubilized with papain, consisted of polypeptides of 23,000 and 30,000 daltons (p23, 30). Rabbit antisera to this material precipitated from [35S]methionine-labeled detergent- solubilized cells, three proteins of 39,000, 34,000, and 29,000 daltons. These antisera were specifically cytotoxic for B lymphocytes of peripheral blood, for B-lymphoblast cell lines, and for EAC rosette receptor-positive surface Ig-negative (Null) lymphocytes. The p23,30 complex was not present on T lymphocytes, EAC rosette receptor-negative Null lymphocytes, or platelets. In addition, the p23,30 complex from several cell lines inhibited alloantisera from multiparous Amish women which had been shown to recognize non-HL-A, B-lymphocyte antigens. Some other properties of the anti-p23,30 sera antisera were described.
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This article has been cited by other articles:

• Uhr, J., Capra, J., Vitetta, E., Cook, R. (1979). Organization of the immune response genes. Science 206: 292-297 [Abstract]  
• Springer, T. A., Kaufman, J. F., Siddoway, L. A., Giphart, M., Mann, D. L., Terhorst, C., Strominger, J. L. (1977). Chemical and Immunological Characterization of HL-A-linked B-lymphocyte Alloantigens. Cold Spring Harb Symp Quant Biol 41: 387-396 [Abstract]  

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