Ontogeny of B cells in the chicken. I. Sequential development of clonal diversity in the bursa

doi:10.1084/jem.144.1.79

This Article

	Full Text (PDF, 1699K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lydyard, P. M.
	Articles by Cooper, M. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lydyard, P. M.
	Articles by Cooper, M. D.


Social Bookmarking

	What's this?

ARTICLES

Ontogeny of B cells in the chicken. I. Sequential development of clonal diversity in the bursa

PM Lydyard, CE Grossi and MD Cooper

The initial development and distribution of lymphocytes expressing surface IgM (sIgM) and of specific antigen-binding cells (ABC) were studied in the chicken in an attempt to gain information on the process by which B-cell diversity is generated. The antigens used were sheep erythrocytes (SE), keyhole limpet hemocyanin (KLH), and poly-L(Tyr, Glu)poly-D,L-Ala-poly-L-Lys (TGAL). The results indicate that generation of the total sIgM-positive population begins in the bursa and that specific clones of ABC develop in a fixed sequential pattern which is not influenced by either deprivation of or deliberate exposure to exogenous antigens. Cells bearing sIgM by immunofluorescence (IgM- positive cells) were detected first in the bursa on the 12th day of incubation, KLH-ABC and TGAL-ABC by the 16th day, and SE-ABC on the 18th day. The doubling time of the sIgM-positive population of bursal cells was determined to be approximately 10 h before significant antigen-independent seeding to the spleen began a few days before hatching. Clonal expansion of SE-ABC in the bursa also appeared to be antigen independent as was the initial development of SE-ABC in the blood and spleen which ceased abruptly after bursectomy at hatching. Specific ABC were observed to develop in multiple bursal follicles as small foci of ABC among the much larger total population of sIgM- positive cells within an individual follicle. Intravenously infused SE- ABC homed to the embryonic spleen but not to the bursa. The results are interpreted as favoring a hypothetical model in which individual stem cells give rise to multiple clones of B cells by a predetermined pattern of sequential expression of variable region genes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Dibner, J. J., Richards, J. D., Knight, C. D. (2008). Microbial Imprinting in Gut Development and Health. J. Appl. Poult. Res. 17: 174-188 [Abstract] [Full Text]
Butler, J. E., Weber, P., Sinkora, M., Sun, J., Ford, S. J., Christenson, R. K. (2000). Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus. J. Immunol. 165: 6999-7010 [Abstract] [Full Text]
Sayegh, C. E., Ratcliffe, M. J. H. (2000). Perinatal Deletion of B Cells Expressing Surface Ig Molecules That Lack V(D)J-Encoded Determinants in the Bursa of Fabricius Is Not Due to Intrafollicular Competition. J. Immunol. 164: 5041-5048 [Abstract] [Full Text]
Sayegh, C. E., Demaries, S. L., Iacampo, S., Ratcliffe, M. J. H. (1999). Development of B cells expressing surface immunoglobulin molecules that lack V(D)J-encoded determinants in the avian embryo bursa of Fabricius. Proc. Natl. Acad. Sci. USA 96: 10806-10811 [Abstract] [Full Text]
Arakawa, H., Kuma, K.-i., Yasuda, M., Furusawa, S., Ekino, S., Yamagishi, H. (1998). Oligoclonal Development of B Cells Bearing Discrete Ig Chains in Chicken Single Germinal Centers. J. Immunol. 160: 4232-4241 [Abstract] [Full Text]
Masteller, E., Larsen, R., Carlson, L., Pickel, J., Nickoloff, B, Lowe, J, Thompson, C., Lee, K. (1995). Chicken B cells undergo discrete developmental changes in surface carbohydrate structure that appear to play a role in directing lymphocyte migration during embryogenesis. Development 121: 1657-1667 [Abstract]
McCormack, W T, Tjoelker, L W, Barth, C F, Carlson, L M, Petryniak, B, Humphries, E H, Thompson, C B (1989). Selection for B cells with productive IgL gene rearrangements occurs in the bursa of Fabricius during chicken embryonic development.. Genes Dev. 3: 838-847 [Abstract]
Alt, F., Blackwell, T., Yancopoulos, G. (1987). Development of the primary antibody repertoire. Science 238: 1079-1087 [Abstract]
Weill, J., Reynaud, C. (1987). The chicken B cell compartment. Science 238: 1094-1098 [Abstract]
Cooper, M. D., Kearney, J. F., Lydyard, P. M., Grossi, C. E., Lawton, A. R. (1977). Studies of Generation of B-cell Diversity in Mouse, Man, and Chicken. Cold Spring Harb Symp Quant Biol 41: 139-145 [Abstract]