An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes

doi:10.1084/jem.143.6.1562

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An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes

MA Skinner and J Marbrook

The cell-mediated immune response has been generated in vitro with a polyacrylamide culture system which allows the segregation of foci (clones?) of cytotoxic lymphocytes. Using the method of limiting dilutions, the frequency of precursor cells in CBA spleen cells able to generate a cytotoxic response against DBA mastocytoma is estimated at 1 per 1,700 cells.
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