Journal of Experimental Medicine, Vol 143, 1382-1390, Copyright © 1976 by Rockefeller University Press

ARTICLES

Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses

J Jandinski, H Cantor, T Tadakuma, DL Peavy and CW Pierce

Concanavalin A, a nonspecific polyclonal activator of T lymphocytes, activates Lyl and Ly23 subclasses to the same degree. After activation, the Ly23 subclass, but not the Lyl subclass, has the following properties: (a) Suppression of the antibody response to sheep erythrocytes (SRBC) in vitro. (b) Production of a soluble factor that suppresses the anti-SRBC response in vitro. (c) Suppression of the generation of cell-mediated cytotoxicity to H-2 target cells in vitro. Con A-activated cells of the Lyl subclass, but not the Ly23 subclass, express helper function in the anti-SRBC response in vitro. Because the intact Con A-stimulated T-cell population contains both cell types, these cells do not exert detectable helper effects in an anti-SRBC system in vitro, because the helper effect of Lyl cells is masked by the suppressor effect of the Ly23 cells. Each function is revealed by eliminating one or the other population with the relevant Ly antiserum. The resting T-cell population, before activation by Con A, also contains already programmed Lyl and Ly23 cells with similar helper and suppressor potentials, respectively. This is revealed by experiments with Ly subclasses which have been separated from the resting T-cell population and then stimulated by Con A. Thus helper and suppressor functions, as expressed in these systems, are manifestations of separate T-cell-differentiative pathways and do not depend upon stimulation of the cells by antigen.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Kapp, J. A., Honjo, K., Kapp, L. M., Xu, X. y., Cozier, A., Bucy, R. P. (2006). TCR transgenic CD8+ T cells activated in the presence of TGF{beta} express FoxP3 and mediate linked suppression of primary immune responses and cardiac allograft rejection. Int Immunol 18: 1549-1562 [Abstract] [Full Text]  
• Chess, L. (2006). The Birth of Functionally Distinct T Cell Subsets. J. Immunol. 176: 3859-3860 [Full Text]  
• Jiang, H., Kashleva, H., Xu, L.-X., Forman, J., Flaherty, L., Pernis, B., Braunstein, N. S., Chess, L. (1998). T cell vaccination induces T cell receptor Vbeta -specific Qa-1-restricted regulatory CD8+ T cells. Proc. Natl. Acad. Sci. USA 95: 4533-4537 [Abstract] [Full Text]  
• Miller, C. L. (1978). Immunological Assays as Measurements of Nutritional Status: A Review. JPEN J Parenter Enteral Nutr 2: 554-566  
• Paul, W., Benacerraf, B (1977). Functional specificity of thymus- dependent lymphocytes. Science 195: 1293-1300 [Abstract]  
• Cantor, H., Boyse, E. A. (1977). Regulation of Cellular and Humoral Immune Responses by T-cell Subclasses. Cold Spring Harb Symp Quant Biol 41: 23-32 [Abstract]  
• Gershon, R. K., Eardley, D. D., Naidorf, K. F., Ptak, W. (1977). The Hermaphrocyte: A Suppressor-Helper T Cell. Cold Spring Harb Symp Quant Biol 41: 85-91 [Abstract]  
• Vitetta, E. S., Cambier, J., Forman, J., Kettman, J. R., Yuan, D., Uhr, J. W. (1977). Immunoglobulin Receptors on Murine B Lymphocytes. Cold Spring Harb Symp Quant Biol 41: 185-191 [Abstract]  
• Shreffler, D. C., David, C. S., Cullen, S. E., Frelinger, J. A., Niederhuber, J. E. (1977). Serological and Functional Evidence for Further Subdivision of the I Regions of the H-2 Gene Complex. Cold Spring Harb Symp Quant Biol 41: 477-487 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS