Journal of Experimental Medicine, Vol 143, 861-869, Copyright © 1976 by Rockefeller University Press

ARTICLES

Idiotypic analysis of lymphocytes in vitro. II. Genetic control of T- helper cell responsiveness to anti-idiotypic antibody

GJ Hammerling, SJ Black, C Berek, K Eichmann and K Rajewsky

When the IgG1 fraction of anti-idiotypic antibodies raised in guinea pigs is injected into mice, sensitization of idiotypic T and B lymphocytes occurs (1-3). In the present study we analyze the genetic requirements for T-helper cell sensitization by anti-idiotypic antibody. This was done by measuring, in a suitable panel of mouse strains, helper cell responsiveness to two anti-idiotypic reagents which recognize distinct, strain-specific idiotypes, namely the A5A and the S117 marker. Whenever helper cell sensitization by anti-idiotypic antibody was successful, helper function could be specifically inhibited by the same and only the same anti-idiotype. This indicates that helper cells induced by anti-idiotypic antibody express idiotypic determinants on their receptors for antigen. Helper cell sensitization by anti-idiotypic antibody was found in all strains expressing the corresponding or a cross-reactive idiotype at the immunoglobulin level. Idiotype-negative strains were always unresponsive to anti-idiotypic stimulation. In addition, responsiveness did not depend on the H-2 haplotype. Since the A5A and the S117 idiotype are markers for V genes in the heavy-chain linkage group, the present results support the view that the same genes in the Ig-1 complex code for variable portions of immunoglobulins and T-helper cell receptors.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Binz, H., Wigzell, H. (1977). Antigen-binding, Idiotypic Receptors from T Lymphocytes: An Analysis of Their Biochemistry, Genetics, and Use as Immunogens To Produce Specific Immune Tolerance. Cold Spring Harb Symp Quant Biol 41: 275-284 [Abstract]  
• Krawinkel, U., Cramer, M., Berek, C., Hammerling, G., Black, S. J., Rajewsky, K., Eichmann, K. (1977). On the Structure of the T-cell Receptor for Antigen. Cold Spring Harb Symp Quant Biol 41: 285-294 [Abstract]  
• Swain, S. L., Trefts, P. E., Tse, H. Y.-S., Dutton, R. W. (1977). The Significance of T-B Collaboration across Haplotype Barriers. Cold Spring Harb Symp Quant Biol 41: 597-609 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS